Human ZNRF3 protein, His Tag (MALS verified)
Synonym
ZNRF3, Zinc, RING finger protein 3, RING-type E3 ubiquitin transferase ZNRF3, RING finger protein 203, KIAA1133, RNF203
Source
Human ZNRF3, His Tag (ZN3-H52H3) is expressed from human 293 cells (HEK293). It contains AA Lys 56 - Met 219 (Accession # Q9ULT6-1).
Predicted N-terminus: Lys 56
Molecular Characterization
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 19.7 kDa. The protein migrates as 22-25 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 1.0 EU per μg by the LAL method / rFC method.
Purity
>95% as determined by SDS-PAGE.
>95% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.
Background
The transmembrane E3 ubiquitin ligase zinc and ring finger 3 (ZNRF3) is a negative feedback regulator of Wnt signalling. ZNRF3 is associated with the Wnt receptor complex, and inhibits Wnt signalling by promoting the turnover of frizzled and LRP6 and acts as a tumor suppressor in the intestinal stem cell zone by inhibiting the Wnt signaling pathway.
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