IdeS (70U/μl)

Product Details

This product is derived from Streptococcus pyogenes and expressed in E. coli, it is an IgG-specific cysteine protease that digests antibodies at a single amino acid site below the hinge, generating homogenous F(ab’)2 and Fc fragments.

Application

IdeS cleaves IgG to produce F(ab')2 and Fc fragments at specific site.

Unit Definition

One unit digests ≥ 95% of 1 μg human IgG when incubated in 10 mM sodium phosphate, 137 mM NaCl, 2.7 mM KCl, pH7.4 at 37°C for 30min.

Purity

>95% as determined by SDS-PAGE.

Enzyme Activity

>70 U/μL

Endotoxin

Less than 1.0 EU per μg by the LAL method / rFC method.

Formulation

Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, PH7.5 with trehalose as protectant.

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Storage

This product is stable after storage at: