Human Integrin alpha 1 beta 1 (ITGA1&ITGB1) Heterodimer Protein, His Tag&Tag Free
Synonym
Integrin alpha 1 beta 1, ITGA1&ITGB1
Source
Human ITGA1&ITGB1 Heterodimer Protein, His Tag&Tag Free (IT1-H52Wb) is expressed from human 293 cells (HEK293). It contains AA Phe 29 - Pro 1141 (ITGA1) & Gln 21 - Asp 728 (ITGB1) (Accession # P56199-1 (ITGA1) & P05556-1 (ITGB1)).
Predicted N-terminus: Phe 29 (ITGA1) & Gln 21 (ITGB1)
Molecular Characterization
Human ITGA1&ITGB1 Heterodimer Protein, His Tag&Tag Free, produced by co-expression of ITGA1 and ITGB1, has a calculated MW of 130.0 kDa (ITGA1) and 83.7 kDa (ITGB1). Subunit ITGA1 is fused with an acidic tail at the C-terminus and followed by a polyhistidine tag and subunit ITGB1 contains no tag but a basic tail at the C-terminus. The protein migrates as 170-200 kDa (ITGA1) and 90-105 kDa (ITGB1) when calibrated against Star Ribbon Pre-stained Protein Marker under non-reducing (NR) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 1.0 EU per μg by the LAL method / rFC method.
Purity
>95% as determined by SDS-PAGE.
Formulation
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH7.5 with trehalose as protectant.
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Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.
Background
Integrin alpha-1/beta-1 is a receptor for laminin and collagen. The Integrin alpha-1/beta-1 and Integrin alpha-2/beta-1 integrins provide critical support for vascular endothelial growth factor signaling, endothelial cell migration, and tumor angiogenesis. It recognizes the proline-hydroxylated sequence G-F-P-G-E-R in collagen. Involved in anchorage-dependent, negative regulation of EGF-stimulated cell growth.
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