| Name | JW 55 |
| Description | JW 55 (JW55) is an effective and selective β-catenin signaling pathway inhibitor, works by inhibition of the PARP domain of tankyrase 1 and tankyrase 2 (TNKS1/2). |
| Cell Research | JW 55 (JW55) is prepared in DMSO and stored, and then diluted with appropriate medium[1]. A total of 1,000 SW480 or RKO cells are seeded in 96-well plates. The day after, the cell culture medium is exchanged to solutions that contained 0.1% DMSO or 10 μM JW55 for RKO cells and 0.1% DMSO or 10, 5, or 1 μM JW55 for SW480 cells. All samples consist of a minimum of 6 replicates. The plate is incubated in an IncuCyte inside a cell culture incubator. Images are captured every second hour to monitor proliferation[1]. |
| In vitro | JW 55 (JW55) is a potent and selective inhibitor of the canonical Wnt pathway. Wnt3a-induced HEK293 cells containing a transiently transfected ST-Luc (SuperTop-luciferase) reporter show inhibition by JW55 with an IC50 value of 470 nM. JW55 is effective in the range of 1 to 5 μM in SW480 cells and 0.01 to 5 μM in HCT-15 cells. JW55 is effective in the range of 1 to 5 μM in SW480 cells and 0.01 to 5 μM in HCT-15 cells[1]. |
| In vivo | JW 55 (100 mg/kg, orally) reduces tumor development in conditional Apc knockout mice. JW55 reduces XWnt8-induced axis duplication inXenopus embryos and Tamoxifen-induced polyposis formation in conditional APC mutant mice[1]. |
| Storage | Shipping with blue ice/Shipping at ambient temperature. |
| Solubility Information | 10% DMSO+40% PEG300+5% Tween 80+45% Saline : 2 mg/mL (4.6 mM), Sonication is recommended.
Ethanol : 100 mM, Sonication is recommended.
DMSO : 50 mg/mL (115.08 mM), Sonication is recommended.
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| Keywords | βcatenin | Wnt/β-catenin | Wnt/betacatenin | Wnt/b-catenin | Wnt | TNKS2 | TNKS1 | poly ADP ribose polymerase | PARP | JW-55 | JW 55 | Inhibitor | inhibit | beta-catenin | betacatenin | bcatenin |
| Inhibitors Related | Chlorquinaldol | Urea | BGP-15 | Neohesperidin | 3-Aminobenzamide | Ethyl linoleate | Monensin sodium salt | Niraparib | AZ9482 | Olaparib | Benzamide | Methyl Vanillate |
| Related Compound Libraries | Reprogramming Compound Library | Neural Regeneration Compound Library | Anti-Cancer Metabolism Compound Library | Hematonosis Compound Library | Anti-Breast Cancer Compound Library | Inhibitor Library | Neuroprotective Compound Library | Stem Cell Differentiation Compound Library | Anti-Prostate Cancer Compound Library | Anti-Aging Compound Library | Anti-Metabolism Disease Compound Library | Neuronal Differentiation Compound Library |
United States
