| Name | KN-62 |
| Description | KN-62 is a potent and specific Ca2+/calmodulin-dependent protein kinase II (CaMKII) inhibitor with Ki of 0.9 μM. |
| Cell Research | For cell growth analysis, K562 cells are plated in a 3-cm dish with 5 mL of culture medium containing various concentration of KN-62. After two days in these condition cell numbers are counted. (Only for Reference) |
| Kinase Assay | Kinase assay: Total kinase activity of CaMKII, determined in a standard 2 min assay (100 μL), contained 35 mM HEPES, 10 mM MgCl2, 1 mM CaCl2, 10 μg of chicken gizzard myosin 20-kD light chain, 0.1 μM calmodulin, and 10 μM [γ-33]ATP at 30 °C. The kinase reaction is halted by adding 1 mL of 10% trichloroacetic acid. |
| In vitro | KN-62 administration in adult rats reduces the expression levels of brain-derived neurotrophic factor (BDNF) mRNA induced by epilepsy in the brain. |
| In vivo | KN-62 inhibits the proliferation of K562 cells and blocks cell cycle progression. When administered at 10 μM to rat pancreatic islet cells, KN-62 inhibits insulin secretion stimulated by carbachol and potassium. Furthermore, KN-62 suppresses the phosphorylation of Ca2+/CaM kinase induced by A23187 in PC12 D cells. |
| Storage | Shipping with blue ice/Shipping at ambient temperature. |
| Solubility Information | 10% DMSO+40% PEG300+5% Tween 80+45% Saline : 2 mg/mL (2.77 mM), Sonication is recommended.
DMSO : 125 mg/mL (173.17 mM), Sonication is recommended.
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| Keywords | P2XRs | P2XReceptor | P2X Receptor | noncompetitive antagonism | KN-62 | KN62 | KN 62 | Inhibitor | inhibit | HEK293 cells | CaMKII | CaMKⅤ | CaMK | Calmodulin-dependent protein kinases | Calmodulin-dependent kinases | autophosphorylation | Autophagy |
| Inhibitors Related | Stavudine | Aceglutamide | Hemin | Tamoxifen | Cysteamine hydrochloride | Guanidine hydrochloride | Hydroxychloroquine | Enzalutamide | Paeonol | Naringin | Alginic acid | Sildenafil citrate |
| Related Compound Libraries | Bioactive Compound Library | Neuronal Signaling Compound Library | Membrane Protein-targeted Compound Library | Kinase Inhibitor Library | Autophagy Compound Library | Post-Translational Modification Compound Library | Inhibitor Library | NO PAINS Compound Library | Bioactive Compounds Library Max | Covalent Inhibitor Library | Ion Channel Targeted Library | Anti-Cancer Compound Library |
United States
