| Name | L 012 sodium salt |
| Description | L 012 sodium salt, a luminol analogue, is a widely used chemiluminescent (CL) probe for active oxygen and active nitrogen. L 012 sodium salt is widely used to detect the superoxides (O2•−) derived from NADPH oxidase (Nox) to find Nox inhibitors. |
| In vitro | L 012 sodium salt is a new chemiluminescent probe. In activated EoL-1 cells which generated reactive oxygen species (ROS), treatment with L-012 generated a significant chemiluminescence. While, superoxide dismutase (100-300 U/ml) abolished the chemiluminescence, which suggested that the main ROS detected was O2- [1]. In cultured endothelial cells, phorbol 12-myristate 13-acetate (PMA, 1 μM) caused an 2.8-fold increase of L-012 chemiluminescence. Vanadate (100 μM) increased the chemiluminescence up to 17-fold. These results suggested that the increase of superoxide produced by NAD(P)H oxidase |
| In vivo | In the human oral cavity and blood and rat peritoneal cavity, L-012 reacted with ROS generated by activated neutrophils and generated strong chemiluminescence, which was higher than that of MCLA [2].
In nonobese diabetic mice and K8 knockout (K8(-/-)) mice, treatment with dextran sulfate sodium generated an increased L-012 chemiluminescence which detected reactive oxygen and nitrogen species (ROS and RNS) [3]. |
| Storage | Shipping with blue ice/Shipping at ambient temperature. |
| Solubility Information | H2O : 3.16 mg/mL (10.17 mM), Sonication and heating to 60℃ are recommended.
10% DMSO+40% PEG300+5% Tween 80+45% Saline : 1 mg/mL (3.22 mM), Sonication is recommended.
DMSO : 45 mg/mL (144.85 mM), Sonication is recommended.
|
| Keywords | L 012 sodium salt | L 012 sodium |
| Inhibitors Related | Ethoxyquin | Kaolin | Allopurinol | Cysteamine hydrochloride | Ethyl linoleate | Magnesium acetate tetrahydrate | Inosine | L-Cystine | Copper Sulfate Pentahydrate | Dimethyl itaconate | L-Ascorbic acid sodium salt | Dimethyl phthalate |
| Related Compound Libraries | NF-κB Signaling Compound Library | Bioactive Compound Library | Immunology/Inflammation Compound Library | Bioactive Compounds Library Max |
United States
