Human Laminin 332 Protein, premium grade
Product Details
Laminin 332 is indispensable for the efficient and specific differentiation of human induced pluripotent stem cells (iPSCs) into corneal epithelial cells. This laminin isoform is a fundamental component of the corneal epithelial basement membrane in vivo, where it provides not just structural anchorage but also essential pro-differentiation signals. When used as a coating substrate in vitro, LN332 mimics this native niche, powerfully directing iPSCs towards the corneal epithelial lineage. It achieves this by engaging with cellular integrins (primarily α6β4), which triggers signaling pathways that suppress alternative cell fates and actively promote the expression of key corneal epithelial markers like KRT3 and KRT12. This makes LN332 a critical tool for generating authentic, functional corneal epithelial cells for applications in disease modeling, drug testing, and the development of regenerative therapies for ocular surface disorders.
Key parameter
Purity (SDS PAGE) | > 95% |
Mycoplasma Test | Negative |
Sterility Test | Negative |
Endotoxin Test | <0.01 EU per μg |
Host Cell Protein | <0.5 ng/µg |
Protein A | <5 ppm |
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 24 months in lyophilized state;
-70°C for 12 months under sterile conditions after reconstitution.
China
