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ChemicalBook Product Catalog LY 303511

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LY 303511 NEW

$330.00 25mg

$562.00 50mg

$898.00 100mg

Min. Order
Purity99.91%
Cas No154447-38-8
Supply Ability10g
Update time2026-04-13

TargetMol Chemicals Inc.

TargetMol Chemicals Inc.

VIP2Y

United States

United States

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Chemical Properties

Product Name	LY 303511
CAS No	154447-38-8
EC-No
Min. Order
Purity	99.91%
Supply Ability	10g
Release date	2026/04/13

Product Introduction

Bioactivity

Name	LY 303511
Description	LY303511, an inactive analogue of LY294002, is a mTOR inhibitor and no inhibition for PI3-K.
Kinase Assay	Mammalian DNA-PK (500 ng/μL) is isolated from HeLa cell nuclear extract after chromatography using Q-Sepharose, S-Sepharose, and Heparin agarose. DNA-PK (250 ng) activity is measured at 30°C, in a final volume of 40 μL, in buffer containing 25 mM HEPES (pH 7.4), 12.5 mM MgCl2, 50 mM KCl, 1 mM DTT, 10% v/v Glycerol, 0.1% w/v NP-40, and 1 mg of the substrate GST-p53N66 (the NH2-terminal 66 amino acid residues of human wild-type p53 fused to glutathione S-transferase) in polypropylene 96-well plates. To the assay mix, varying concentrations of inhibitor (in DMSO at a final concentration of 1% v/v) are added. After 10 min of incubation, ATP is added to give a final concentration of 50 μM, along with a 30-mer double-stranded DNA oligonucleotide (final concentration of 0.5 ng/mL), to initiate the reaction. After 1 h with shaking, 150 μL of PBS are added to the reaction, and 5 μL are then transferred to a 96-well opaque white plate containing 45 μL of PBS per well, where the GSTp53N66 substrate is allowed to bind to the wells for 1 h. To detect the phosphorylation event on the serine 15 residue of p53 elicited by DNA-PK, a p53 phosphoserine-15 antibody is used in a basic ELISA procedure. An antirabbit horseradish peroxidase-conjugated secondary antibody is then used in the ELISA before the addition of chemiluminescence reagent to detect the signal as measured by chemiluminescent counting via a TopCount NXT[1].
Storage	Shipping with blue ice/Shipping at ambient temperature.
Solubility Information	DMSO : 100 mM, Sonication is recommended.
Keywords	Tumor Necrosis Factor Receptor \| TNFR \| TNF Receptor \| PotassiumChannel \| Potassium Channel \| mTOR \| LY-303511 \| LY303511 \| LY 303511 \| KcsA \| Inhibitor \| inhibit
Inhibitors Related	Kaolin \| Undecane \| Fluorene \| Acetylcysteine \| Ethyl palmitate \| Gum arabic \| 1,8-Cineole \| Dodecane \| 1,4-Naphthoquinone \| Tributyrin \| Glucosamine \| Methyl eugenol

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