| Name | MCB-613 |
| Description | MCB-613 is an effective steroid receptor coactivator (SRC) stimulator. |
| Cell Research | Cells are seeded in 96-well plates and allowed to reach 60% to 70% confluence. After indicated compound treatments, relative numbers of viable cells are measured by MTS assay using the Cell Titer 96 Aqueous One Solution Cell Proliferation Assay.(Only for Reference) |
| Kinase Assay | Luciferase assays: After various compound treatments, cells are lysed in luciferase lysis buffer and assayed for luciferase activity using the ONE-Glo luciferase assay system. All luciferase activities are normalized to protein concentration determined by Bradford assay. |
| In vitro | MCB-613 selectively and reversibly binds to the RID of SRC-3, and selectively kills cancer cells including MCF-7 (breast), PC-3 (prostate), H1299 (lung), and HepG2 (liver) cells, without toxicity to mouse primary hepatocytes and mouse embryonic fibroblasts (MEFs). MCB-613 also increases SRCs' interactions with other coactivators and markedly induces ER stress coupled to the generation of reactive oxygen species (ROS). [1] |
| In vivo | In an MCF-7 breast cancer mouse xenograft model, MCB-613 (20 mg/kg, i.p.) significantly and dramatically inhibits the growth of the tumor while causing no obvious animal toxicity and body weight less. [1] |
| Storage | keep away from moisture,store at low temperature | Shipping with blue ice/Shipping at ambient temperature. |
| Solubility Information | H2O : < 1 mg/mL (insoluble or slightly soluble)
Ethanol : 56 mg/mL (183.97 mM), Sonication is recommended.
DMSO : 56 mg/mL (183.97 mM), Sonication is recommended.
10% DMSO+40% PEG300+5% Tween 80+45% Saline : 2 mg/mL (6.57 mM), Sonication is recommended.
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| Keywords | Src | MCB-613 | MCB613 | Inhibitor | inhibit |
| Inhibitors Related | Ethoxyquin | Kaolin | Stavudine | Aceglutamide | Allopurinol | Urea | Tamoxifen | Metronidazole | Ethyl linoleate | Formamide | Alginic acid | Sildenafil citrate |
| Related Compound Libraries | NF-κB Signaling Compound Library | Target-Focused Phenotypic Screening Library | Bioactive Compound Library | Endoplasmic Reticulum Stress Compound Library | Membrane Protein-targeted Compound Library | HIF-1 Signaling Pathway Compound Library | Kinase Inhibitor Library | Tyrosine Kinase Inhibitor Library | Mitochondria-Targeted Compound Library | Immunology/Inflammation Compound Library | Bioactive Compounds Library Max | Anti-Cancer Active Compound Library |
United States
