Biotinylated Monoclonal Anti-FMC63 Antibody, Mouse IgG1, Avitag™ (Y45)
Source
Monoclonal Anti-FMC63 Antibody, Mouse IgG1, Avitag™ (Y45) is a neutralizing mouse monoclonal antibody recombinantly expressed from human 293 cells (HEK293). Biotinylation of this antibody is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
Application
Flow Cytometry (Evaluation of Anti-CD19 (FMC63 scFv) CAR Expression).
Clone
Y45
Species
Mouse
Isotype
Mouse IgG1 & | kappa
Specificity
Specifically recognizes the antigen-recognition domain of FMC63 derived CARs.
Immunogen
Recombinant FMC63 scFv derived from HEK293 cells
Conjugate
Biotin
Isotype Control
The Isotype control is sold separately and you can search for Cat. No. DNP-BM190 for product information.
Recommended Dilution
1:50
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, 0.5% BSA, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
1. -20°C to -70°C for 24 months in lyophilized state;
2. -70°C for 12 months under sterile conditions after reconstitution.
Background
FMC63 is an IgG2a mouse monoclonal antibody specific for CD19, which is a target for the immunotherapy of B lineage leukaemias and lymphomas. FMC63 scFv is the most commonly used ectodomain component of CD19-specific CARs. So far, most of reported CART19 trials contain the anti-CD19 scFv derived from FMC63, including the two FDA-approved CARs Kymriah and Yescarta.
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