Monoclonal Anti-Human-IgG-Fc Antibody

Source

Monoclonal Anti-Human-IgG-Fc Antibody, Mouse IgG1 is a Mouse monoclonal antibody recombinantly expressed from CHO cells.

Clone

6F11C8

Species

Mouse

Isotype

Mouse IgG1 | Mouse Kappa

Conjugate

Unconjugated

Antibody Type

Recombinant Monoclonal

Reactivity

Human IgG Fc, including IgG1–IgG4 isotypes and Fc-engineered variants (e.g. N297A, LALA, YTE, LS, S239D/I332E)

Immunogen

Recombinant Human Fc derived from CHO cells

Specificity

Specifically binds to the Fc region of human IgG without recognizing Fab fragments or mouse IgG isotypes.

Application

Purity

95% as determined by SDS-PAGE.

90% as determined by SEC-MALS.

Purification

Protein A purified / Protein G purified

Formulation

Lyophilized from a 0.22 μm-filtered solution in PBS, with trehalose as protectant.

Please contact us for customized product forms or formulations.

Reconstitution

Please refer to the Certificate of Analysis (CoA) for specific instructions.

For best performance, we strongly recommend following the reconstitution protocol provided in the CoA.

Storage

For long term storage, the product should be stored in a lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

Cross Verification

Human Fc recognition This antibody recognizes all four human IgG isotypes (IgG1, IgG2, IgG3, IgG4) and multiple Fc-engineered variants, including N297A, LALA, YTE, LS, S239D/I332E, etc.
Species cross-reactivity
• Weak cross-reactivity with cynomolgus IgG was observed by ELISA.
• No detectable binding to mouse IgG isotypes (IgG1, IgG2a, IgG2b).