| Name | NAD+ |
| Description | NAD+ (β-Nicotinamide Adenine Dinucleotide) is a coenzyme composed of ribosylnicotinamide 5'-diphosphate coupled to adenosine 5'-phosphate by pyrophosphate linkage. It is found widely in nature and is involved in numerous enzymatic reactions in which it serves as an electron carrier by being alternately oxidized (NAD+) and reduced (NADH). |
| In vitro | METHODS: HEK293 cells were treated with FK866 (2 µM) and NAD+ (100 µM) for 48 h. Metabolic activity was determined by MTT Assay.
RESULTS: Addition of FK866 to the culture medium resulted in rapid depletion of intracellular NAD stores and inhibition of the metabolic activity of NADPH-dependent dehydrogenase. When supplemented with additional NAD+, the metabolic activity of the cells returned to control levels. [1]
METHODS: Isolated microvessels from rat retina were treated with NAD+ (0-1000 nM) for 0-24 h. Cell death was detected using trypan blue dye.
RESULTS: Exposure to NAD+ increased microvascular cell death in a dose-dependent manner, with the half-maximum effective concentration of NAD+ being approximately 2 nM. assessment of the time course of NAD+-induced vascular toxicity showed that cell death was detected after 16 h of NAD+ exposure. [2] |
| In vivo | METHODS: To study the effects on ischemia/reperfusion (I/R) injury, NAD+ (5-20 mg/kg) was injected intravenously into Wistar rats with myocardial ischemia/reperfusion.
RESULTS: Injections of 10-20 mg/kg NAD+ dose-dependently reduced I/R-induced myocardial infarction, with a dose of 20 mg/kg NAD+ reducing infarction by approximately 85%. Injection of NAD+ significantly reduced I/R-induced apoptotic cardiac injury. [3] |
| Storage | store at low temperature,keep away from direct sunlight,store under nitrogen | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature. |
| Solubility Information | 5% DMSO+95% Saline : 0.33 mg/mL (0.5 mM), Solution.
DMSO : 6.7 mg/mL (10.1 mM), Sonication is recommended.
PBS : 100 mg/mL (150.73 mM), Sonication is recommended.
H2O : 53.3 mg/mL (80.34 mM), Sonication and heating are recommended.
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| Keywords | NADPH | NAD+ | Inhibitor | inhibit | EndogenousMetabolite | Endogenous Metabolite | b-Nicotinamide Adenine Dinucleotide | b-NAD | beta-Nicotinamide Adenine Dinucleotide | beta-NAD | beta-DPN | b-DPN |
| Inhibitors Related | Sucrose | Aceglutamide | L-Pyroglutamic acid | Nicotinamide riboside malate | D(+)-Raffinose pentahydrate | Guanidine hydrochloride | Formamide | Glycerol | Thymidine | Corn starch | Hyaluronic acid sodium (MW 20 kDa) | Gluconate Calcium |
| Related Compound Libraries | Bioactive Compound Library | ReFRAME Related Library | Selected Plant-Sourced Compound Library | Natural Product Library | Drug Repurposing Compound Library | Anti-Viral Compound Library | Tobacco Monomer Library | Natural Product Library for HTS | Cosmetic Ingredient Compound Library | Human Endogenous Metabolite Library | Bioactive Compounds Library Max | Gut Microbial Metabolite Library |
United States
