| Name | Naphthofluorescein |
| Description | Naphthofluorescein (Naphthafluorescein) is a Mint3 inhibitor with potential antitumor and anti-inflammatory activities, inhibits the interaction between HIF-1 and Mint3, inhibits Mint3-dependent HIF-1 activity, and inhibits glycolytic activity in cancer cells and macrophages without showing significant in vitro cytotoxicity or in vivo side effects. Naphthofluorescein is also a fluorescent pH-sensitive probe for functional Cherenkov imaging. |
| Cell Research | Naphthofluorescein as a pH-sensitive fluorescent probe
Procedure
1. 1 mL of HeLa cell suspension (5 × 104 cells) was seeded in a 35 mm glass-bottom microwell dish (MatTek) and cultured overnight;
2. The cells were gently washed twice with DPBS and treated with Naphthofluorescein-labeled phenol red-free peptide (5 pm) in HEPES-supplemented DMEM containing 1% FBS at 37°C in the presence of 5% CO2 for 2 h;
3. After removing the culture medium, the cells were gently rinsed twice with DPBS and imaged on a live cell imaging confocal microscope equipped with a 60x oil objective.
The above information is based on published literature. Experimental procedures should be appropriately modified to meet specific research demands. |
| In vitro | Naphthofluorescein (compound 19) (0-10 μM; 24 h; HT1080 cells) significantly suppresses HIF-1 reporter activity in a concentration-dependent manner.[1] |
| In vivo | Naphthofluorescein (compound 19) (100 mg/kg; i.p.; once daily; 5 days followed by 2 days off for 2 weeks; Male C57BL/6J mice) significantly suppresses tumour growth in subcutaneous E0771 cells and attenuates tumour growth in MDA-MB-231 and AsPC-1 cells in immunodeficient mice. It inhibits tumour growth in human cancer cells in an FIH-1-dependent manner and does not affect FIH-1-depleted MDA-MB-231 cells. No weight loss, histological abnormalities in the lung, liver, and kidney, or severe adverse effects were observed in the mice for at least 2 weeks.[1] |
| Storage | Powder: -20°C for 3 years | In solvent: -80°C for 1 year
Shipping with blue ice/Shipping at ambient temperature. |
| Solubility Information | Ethanol : 2 mg/mL (4.63 mM), Sonication is recommended.
DMSO : 100 mg/mL (231.26 mM), Sonication is recommended.
DMF : 10 mg/mL (23.13 mM), Sonication is recommended.
DMF:PBS (pH 7) (1:10) : 0.09 mg/mL (0.21 mM), Sonication is recommended.
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| Keywords | Naphthofluorescein | HIF-1 |
| Inhibitors Related | Deferoxamine Mesylate | Hydralazine hydrochloride | Dapagliflozin | Chlorogenic Acid | Glucosamine | Hydroxycitric acid tripotassium hydrate | Glucosamine hydrochloride | Chloramphenicol | Oroxylin A | Acriflavine Hydrochloride | Albendazole | Glucosamine sulfate |
| Related Compound Libraries | Glycometabolism Compound Library | Bioactive Compound Library | Epigenetics Compound Library | HIF-1 Signaling Pathway Compound Library | Angiogenesis related Compound Library | Inhibitor Library | Immunology/Inflammation Compound Library | Anti-Aging Compound Library | Bioactive Compounds Library Max | Anti-Cancer Compound Library | Anti-Cancer Active Compound Library | Transcription Factor-Targeted Compound Library |
United States
