nitrocefin

nitrocefin is a chromogenic β -lactamase substrate that changes color from yellow to red when the amide bond in the β -lactamase ring is hydrolyzed by β -lactamase.  nitrocefin undergoes color changes induced by lactamide Chemicalbook enzymes produced by gram-positive and Gram-negative bacteria.  Several studies have used the color-changing properties of ncephalosporin to detect β -lactamase activity in bacterial cell extracts by isoelectric focusing and spectroscopy.  nitrocefin has also been used in studies involving β -lactamase resistant antibiotics.