| Name | NPC 15199 |
| Description | NPC 15199 (FMOC-L-Leucine) is an anti-inflammatory agent. |
| In vitro | NPC-15199 [(N-(9-fluorenylmethoxycarbonyl)L-leucine)], a novel anti-inflammatory agent, increases intracellular Ca2+ concentration ([Ca2+]i) in human bladder female transitional cancer (BFTC) cells.?Using fura-2 as a Ca2+ probe, NPC-15199 (0.1-2 mM) was found to increase [Ca2+]i concentration-dependently.?The response saturated at 2-5 mM NPC-15199.?The [Ca2+]i increase comprised an initial rise, a slow decay, and a plateau.?Ca2+ removal partly inhibited the Ca2+ signals.?In Ca2+-free medium, pretreatment with 1 mM NPC-15199 abolished the [Ca2+]i increase induced by 1 microM thapsigargin (an endoplasmic reticulum Ca2+ pump inhibitor);?and after pretreatment with thapsigargin, NPC-15199-induced Ca2+ release was dramatically inhibited.?This indicates that NPC-15199 released internal Ca2+ mostly from the endoplasmic reticulum.?Adding 3 mM Ca2+ increased [Ca2+]i in cells pretreated with 1 mM NPC-15199 in Ca2+-free medium.?Together, the findings suggest that in BFTC bladder cancer cells, NPC-15199 induced Ca2+ release from the endoplasmic reticulum and activating Ca2+ entry. |
| Storage | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature. |
| Solubility Information | DMSO : 25 mg/mL (70.74 mM), Sonication is recommended.
10% DMSO+40% PEG300+5% Tween 80+45% Saline : 2 mg/mL (5.66 mM), Sonication is recommended.
|
| Keywords | PPAR | Peroxisome proliferator-activated receptors | NSC-334290 | NSC334290 | NSC 334290 | NPC-15199 | NPC15199 | NPC 15199 | Inhibitor | inhibit | inflammation | Fmoc-leucine |
| Inhibitors Related | PHYTOL | Rosiglitazone | Retinoic acid | Daidzein | Fenofibrate | Magnesium acetate tetrahydrate | Maltitol | Naringenin | D-Arabinopyranose | 2,3-Butanediol | Icariin | Cloxiquine |
| Related Compound Libraries | Nuclear Receptor Compound Library | DNA Damage & Repair Compound Library | Bioactive Compound Library | Anti-Diabetic Compound Library | ReFRAME Related Library | NO PAINS Compound Library | Anti-Aging Compound Library | Immunology/Inflammation Compound Library | Bioactive Compounds Library Max | Transcription Factor-Targeted Compound Library |
United States
