| Name | PHTPP |
| Description | PHTPP is a selective ERβ antagonist. |
| Cell Research | Cells are plated at a density of 3 to 4×10^5 in 1 mL of medium per well in a 24-well plate for cell viability and cAMP analysis. Cells are cultured in Dulbecco's Modified Eagle Medium with PHTPP (1 μM), or ethanol (0.1%) as the vehicle. The incubator is set to an atmosphere of 5% CO2 in air at 37°C, and cultures are allowed to acclimate for at least 24 h. The culture medium is then aspirated and replaced with serum-free DMEM/F12 containing 0.1 μM androstenedione. The cells are collected for intracellular cAMP and to test cell viability. They are for reference only. |
| In vitro | PHTPP acts as a selective antagonist for estrogen receptor beta (ERβ), significantly decreasing follicle-stimulating hormone (FSH)-mediated cyclic AMP (cAMP) production by 80% (p<0.01), without impacting basal cAMP levels. At a concentration of 10^-6 M, PHTPP inhibits estradiol (E2)-stimulated ERβ activity but does not affect E2-induced ERα activity. Furthermore, a high concentration of PHTPP (10^-6 M) marginally enhances class 1 Igf1 mRNA expression and promotes the DPN-induced increase in class 1 Igf1 mRNA expression. |
| Storage | Shipping with blue ice/Shipping at ambient temperature. |
| Solubility Information | 10% DMSO+40% PEG300+5% Tween 80+45% Saline : 2 mg/mL (4.72 mM), Sonication is recommended.
H2O : < 1 mg/mL (insoluble)
Ethanol : 3.96 mg/mL (9.35 mM), Sonication is recommended.
DMSO : 22.73 mg/mL (53.7 mM), Sonication is recommended.
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| Keywords | progestogenReceptor | progestogen Receptor | PHTPP | Inhibitor | inhibit | EstrogenReceptor | Estrogen Receptor/ERR | Estrogen Receptor | ERβ | ERR |
| Inhibitors Related | Tamoxifen | Mifepristone | Estradiol | Astragaloside IV | Bisphenol B | Bisphenol Z | Mequinol | Phthalic acid | Ethisterone | Alpha-Estradiol | Ferrous Bisglycinate | Cholesterol |
| Related Compound Libraries | Nuclear Receptor Compound Library | Bioactive Compound Library | Membrane Protein-targeted Compound Library | ReFRAME Related Library | Anti-Ovarian Cancer Compound Library | Inhibitor Library | NO PAINS Compound Library | Endocrinology-Hormone Compound Library | Bioactive Compounds Library Max | Fluorochemical Library | Anti-Cancer Compound Library | Human Metabolite Library |
United States
