| Name | Pregnanediol |
| Description | Pregnanediol (5Beta-Pregnane-3Alpha,20alpha-Diol) is an inactive metabolite of PROGESTERONE by reduction at C5, C3, and C20 position. Pregnanediol(5Beta-Pregnane-3Alpha,20alpha-Diol) has two hydroxyl groups, at 3-alpha and 20-alpha. It is detectable in URINE after OVULATION and is found in great quantities in the pregnancy urine. |
| Kinase Assay | Gelatin (0.1% (w/v) is added to standard LaemmLi acrylamide polymerization mixture. Tissue extract is mixed 1:2 with sample buffer [250 mM Tris-Cl pH 6.8, 10% (w/v) SDS, 20% (v/v) glycerol, 0.005% (w/v) bromphenol blue]. Serum is diluted 1:10 with electrophoresis buffer (2.5 mM Tris, 20 mM glycine, 0.005% SDS) and mixed 1:2 with sample buffer. Twenty μLs are loaded after 10-min incubation at room temperature without boiling. After electrophoresis at 90 V, the gels are soaked in 2.5% (w/v) Triton X-100, incubated 2 to 3 days at 37°C in gelatin digestion buffer [50 mM Tris-Cl, pH 8.0, 8 mM CaCl2, 10 mM ZnSO2, 0.02% (w/v) NaN3], stained in 0.05% Coomassie blue R-250 in acetic acid/methanol/water (1:4.5:4.5 by volume), destained in 10% acetic acid and 5% methanol, and scanned for lysis band intensity. The lysis band intensity is proportional to gelatinase activity and is quantified densitometrically by using One-Dimensional Scan software. The result, a number between 0.07 and 3.75, is normalized to the protein content by dividing the densitometry result with the relative optical density from the BCA protein assay kit result. The result is used for the analysis as the arbitrary unit. For the total MMP activity results of lysis bands of pro-MMP-9, active MMP-9, pro-MMP-2, and active MMP-2 are added. A protein size marker is used to determine the correct size. |
| Storage | Shipping with blue ice/Shipping at ambient temperature. |
| Solubility Information | DMSO : Slightly soluble
Ethanol : 4.17 mg/mL (13.01 mM), Sonication is recommended.
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| Keywords | urine | progestogenReceptor | progestogen Receptor | progesterone | Pregnanediol | NSC-47462 | NSC47462 | NSC-1612 | NSC1612 | NSC 47462 | NSC 1612 | metabolic product | measure | Inhibitor | inhibit | EstrogenReceptor | Estrogen Receptor | ER | EndogenousMetabolite | Endogenous Metabolite | 5β-Pregnane-3α,20α-Diol | 5b-Pregnane-3a,20a-Diol |
| Inhibitors Related | Sucrose | Aceglutamide | Nicotinamide riboside malate | DL-Lysine | D(+)-Raffinose pentahydrate | Guanidine hydrochloride | Malic acid | Formamide | Glycerol | Thymidine | Corn starch | Gluconate Calcium |
| Related Compound Libraries | Anti-Tumor Natural Product Library | Nuclear Receptor Compound Library | Bioactive Compound Library | Natural Product Library | Endocrinology-Hormone Compound Library | NO PAINS Compound Library | Natural Product Library for HTS | Human Endogenous Metabolite Library | Human Endogenous Metabolite Compound Library Plus | Bioactive Compounds Library Max | Bioactive Lipid Compound Library | Human Metabolite Library |
United States
