| Name | Pritelivir |
| Description | Pritelivir (BAY 57-1293) (BAY 57-1293) is a potent helicase-primase inhibitor with active against HSV-1 and HSV-2 (IC50: 20 nM). |
| Kinase Assay | In vitro procaspase-3 activation: Procaspase-3 is expressed and purified in Escherichia coli. Various concentrations of PAC-1 are added to 90 μL of a 50 ng/mL of procaspase-3 in caspase assay buffer in a 96-well plate, The plate is incubated for 12 hours at 37 °C. A 10 μL volume of a 2 mM solution of caspase-3 peptidic substrate acetyl Asp-Glu-Val-Asp-p-nitroanilide (Ac-DEVD-pNa) in caspase assay buffer is then added to each well. The plate is read every 2 minutes at 405 nm for 2 hours in a Spectra Max Plus 384 well plate reader. The slope of the linear portion for each well is determined, and the relative increase in activation from untreated control wells is calculated. |
| In vitro | In models of lethal disseminated herpes infection in mice and rats, as well as in a mouse model simulating the spread of shingles-like skin disease, BAY57-1293 (administered orally at doses of 0.5/15 mg/kg) exhibited potent antiviral activity. This compound also showed similar efficacy in a mouse model of ocular herpes. |
| In vivo | BAY57-1293 exhibits a dose-dependent direct inhibition of the viral helicase-primase complex's ATPase activity. It also demonstrates significant antiviral activity against acyclovir-resistant herpes simplex virus mutants. Additionally, in green monkey kidney cells, BAY57-1293 reduces the levels of Aβ and P-tau induced by herpes simplex virus type 1. |
| Storage | Shipping with blue ice/Shipping at ambient temperature. |
| Solubility Information | H2O : < 1 mg/mL (insoluble or slightly soluble)
10% DMSO+40% PEG300+5% Tween 80+45% Saline : 2 mg/mL (4.97 mM), Sonication is recommended.
Ethanol : < 1 mg/mL (insoluble or slightly soluble)
DMSO : 245 mg/mL (608.71 mM), Sonication is recommended.
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| Keywords | virus | viral | RNASynthesis | RNA Synthesis | Pritelivir | primase | Inhibitor | inhibit | HSV-2 | HSV-1 | HSV | Herpes simplex virus | herpes | helicase primase | helicase | DNASynthesis | DNA Synthesis | complex | AIC-316 | AIC 316 |
| Inhibitors Related | 5-Fluorouracil | Adenine hemisulfate | Erythromycin thiocyanate | Guanidine hydrochloride | Hexane-1,6-diol | L-Lysine | 1,4-Naphthoquinone | Adenine | Oxyresveratrol | Carbazole | Thymidine | Usnic Acid |
| Related Compound Libraries | Bioactive Compound Library | Anti-Cancer Clinical Compound Library | Drug Repurposing Compound Library | Anti-Viral Compound Library | Inhibitor Library | Immunology/Inflammation Compound Library | Anti-Aging Compound Library | Bioactive Compounds Library Max | Anti-COVID-19 Compound Library | Anti-Cancer Compound Library | Anti-Infection Compound Library | Anti-Cancer Drug Library |
United States
