| Name | Protopine |
| Description | Protopine (Fumarine) is a benzylisoquinoline alkaloid. It has been found to inhibit histamine H1 receptors and platelet aggregation, and acts as an analgesic. |
| Kinase Assay | HT1080 cells are seeded in 24-well plates (2×104 cells per well) and treated with Dorsomorphin in the presence or absence of glucose or 10 mM 2DG for 2 h. HT1080 cells that overexpressed the wild-type and dominant negative AMPKα1 are prepared by transfecting plasmid DNA (pAMPKα1-wt, pAMPKα1-D168A and pcFlag as a control) in 6-well plates, seeding in 24-well plate and treating with UPR inhibitors. Cells are lysed with cell lysis buffer (20 mM Tris-HCl, pH 7.5, 250 mM NaCl, 10% glycerol, 0.5% NP-40, 1 mM EDTA, 1 mM EGTA, 0.2 mM PMSF, 1 μg/mL pepstatin, 0.5 μg/mL leupeptin, 5 mM NaF, 2 mM Na3Vo4, 2 mM β-glycerophosphate, 1 mM DTT). Relative AMPK kinase activity (mean±SD of duplicate determinations) to control sample (vehicle or pcFlag under normal growth conditions) is determined using the CycLex AMPK kinase assay kit[2]. |
| Storage | Shipping with blue ice/Shipping at ambient temperature. |
| Solubility Information | DMSO : 13.08 mg/mL (37.02 mM), Sonication is recommended.
Chloroform 1:15 : Soluble
10% DMSO+40% PEG300+5% Tween 80+45% Saline : 1 mg/mL (2.83 mM), Sonication is recommended.
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| Keywords | ReactiveOxygenSpecies | Reactive Oxygen Species | Protopine | Plateletaggregation | Platelet aggregation | Inhibitor | inhibit | HistamineReceptor | Histamine Receptor | H1 receptor |
| Inhibitors Related | Ethoxyquin | Cysteamine hydrochloride | Meclizine dihydrochloride | L-Ascorbic acid | Famotidine | L-Cystine | Sodium butanoate | L-Ascorbic acid sodium salt | Sodium 2-oxopropanoate | Dimethyl sulfoxide | Coumaran | Alginic acid |
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