| Name | Puromycin dihydrochloride |
| Description | Puromycin dihydrochloride (CL13900 dihydrochloride) is a cinnamamide adenosine antibiotic and an inhibitor of protein synthesis. Puromycin dihydrochloride inhibits protein synthesis by binding to RNA and has antitumor and antitrypanosomal activity. |
| In vitro | METHODS: Human hepatocellular carcinoma cells, HepG2, and primary rat hepatocytes, PRH, were treated with Puromycin dihydrochloride (0-300 µmol/L) for 72 h, and cell growth inhibition was detected by MTT.
RESULTS: Puromycin dihydrochloride dose-dependently inhibited the growth of HepG2 and PRH cells, with IC50s of 1600 μmol/L and 2000 nmol/L, respectively.[1]
METHODS: Human breast cancer cells MDA-MB-231 and MDA-MB-436 were transfected with shRNAs-Puro lentivirus, and after 24 h, the transfected cells were cultured in new medium containing Puromycin dihydrochloride (1 μg/ml) for about 12 days, and then the cells were screened for the success of transfection.
RESULTS: Puromycin dihydrochloride screened the lentivirally transfected cells successfully. [2]
METHODS: Cells transfected with lentivirus were cultured in medium supplemented with Puromycin dihydrochloride (2 μg/mL).
RESULTS: Puromycin dihydrochloride screened and maintained lentivirally successfully transfected cells. [3] |
| In vivo | Puromycin is utilized to select recombinant cells from non-cultured cells. It serves as a tool for studying protein synthesis in many systems. |
| Storage | Shipping with blue ice/Shipping at ambient temperature. |
| Solubility Information | H2O : 92 mg/mL (168.98 mM), Sonication is recommended.
Ethanol : < 1 mg/mL (insoluble or slightly soluble)
DMSO : 255 mg/mL (468.37 mM), Sonication is recommended.
10% DMSO+40% PEG300+5% Tween 80+45% Saline : 5 mg/mL (9.18 mM), Sonication is recommended.
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| Keywords | RNASynthesis | RNA Synthesis | Puromycin Dihydrochloride | Puromycin | Inhibitor | inhibit | DNASynthesis | DNA synthesis | CL-13900 Dihydrochloride | CL13900 Dihydrochloride | CL-13900 | CL13900 | CL 13900 Dihydrochloride | CL 13900 | Bacterial | Antibiotic | 60S ribosome |
| Inhibitors Related | Neomycin sulfate | D(+)-Raffinose pentahydrate | Guanidine hydrochloride | Sulfamethoxazole sodium | Terbinafine hydrochloride | Hydroxychloroquine | Fenpyroximate | Thymidine | Hyaluronic acid sodium (MW 20 kDa) | Dimethyl sulfoxide | Sodium diacetate | Sodium bicarbonate |
| Related Compound Libraries | Anti-Tumor Natural Product Library | Anti-Parasitic Compound Library | Natural Product Library | Saccharide and Glycoside Natural Product Library | Microbial Natural Product Library | Inhibitor Library | Antiparasitic Natural Product Library | Natural Product Library for HTS | Anti-infective Natural Product Library | Anti-Aging Compound Library | Bioactive Compounds Library Max | Anti-Cancer Active Compound Library |
United States
