| Name | Purvalanol A |
| Description | Purvalanol A (NG-60) is an effective and cell-permeable CDK inhibitor with IC50 of 70/4/35/850 nM for cdk2-cyclin A/B/E, and cdk4-cyclin D1, respectively. |
| Cell Research | Cells are seeded at 10000 density in 96-well plates and treated with various concentrations of Purvalanol A (0-100 μM) for 24 h. Cells are exposed to 10 μL of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltet- razolium bromide dye (5 mg/mL) and are incubated at 37℃ for 4 h. In order to solubilize the formazan crystals 100 μL DMSO is added. Absorbance is determined at 570 nm spectrophotometrically.(Only for Reference) |
| In vivo | In MCF-7 cells, Purvalanol A leads to a 50% reduction in cell viability, while MDA-MB-231 cells exhibit lower sensitivity to Purvalanol A, showing a 32% decrease in cell viability. Purvalanol A induces mitochondrial-mediated apoptosis in both MCF-7 and MDA-MB-231 cells. The compound's effect on reducing the viability of these cell lines is dose-dependent. By inhibiting cell cycle progression and the c-Src signaling pathway, Purvalanol A effectively prevents c-Src-mediated transformation and significantly inhibits anchorage-independent growth in certain human cancer cells with upregulated c-Src. Additionally, Purvalanol A markedly suppresses anchorage-dependent growth in HT29 and SW480 human colorectal cancer cells. |
| Storage | Shipping with blue ice/Shipping at ambient temperature. |
| Solubility Information | DMSO : 250 mg/mL (642.86 mM), Sonication is recommended.
Ethanol : 19.5 mg/mL (50.14 mM), Sonication is recommended.
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| Keywords | Purvalanol A | NG60 | NG 60 | Inhibitor | inhibit | Cyclin dependent kinase | CDK4/CyclinD1 | CDK2/CyclinE | CDK2/CyclinA | CDK | Cdc2/CyclinB | Autophagy | Apoptosis |
| Inhibitors Related | Stavudine | Aceglutamide | Hemin | Urea | Tamoxifen | Guanidine hydrochloride | Hydroxychloroquine | Metronidazole | Formamide | Paeonol | Naringin | Alginic acid |
| Related Compound Libraries | Anti-Pancreatic Cancer Compound Library | Bioactive Compound Library | ReFRAME Related Library | Kinase Inhibitor Library | Autophagy Compound Library | Anti-Breast Cancer Compound Library | Inhibitor Library | Anti-Cardiovascular Disease Compound Library | NO PAINS Compound Library | Anti-Aging Compound Library | Bioactive Compounds Library Max | Cell Cycle Compound Library |
United States
