| Name | R-1479 |
| Description | R-1479 (4'-Azidocytidine) is an RdRp inhibitor with an IC50 value of 1.28 μM for HCV RNA replication in the HCV subgenomic replicon system. |
| Kinase Assay | The membrane-associated, native HCV replicase complex is isolated from 2209-23 HCV replicon cells and a derived cell line carrying HCV replicon RNA with an S282T mutation in the NS5B coding sequence. The in vitro replicase assay contain 10 μL of cytoplasmic membrane fraction, 50 mM HEPES (pH 7.5), 10 mM KCl, 10 mM dithiothreitol, 5 mM MgCl2, 20 μg/mL actinomycin D, 1 mM ATP, 1 mM GTP, 1 mM UTP, 30 μCi of [α-33P]CTP (3000 Ci/mmol, 10 mCi/mL), 1 unit/μL SUPERase•In, 10 mM creatine phosphate, and 200 μg/mL creatine phosphokinase in a final volume of 25 μL [1]. |
| In vitro | R-1479 inhibits HCV RNA replication and reduces Renillaluciferase activity of proliferating replicon cells in a dose-dependent manner[1]. R-1479 inhibits RNA synthesis mediated by NS5B with an IC50 value of 320 nM and the RNA polymerase encoded by HCV[2]. |
| Storage | Shipping with blue ice/Shipping at ambient temperature. |
| Solubility Information | DMSO : 90 mg/mL (316.64 mM), Sonication is recommended.
10% DMSO+40% PEG300+5% Tween 80+45% Saline : 3.3 mg/mL (11.61 mM), Sonication is recommended.
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| Keywords | R-1479 | R1479 | R 1479 | HCVProtease | HCV replicon | HCV Protease |
| Inhibitors Related | 5-Fluorouracil | Adenine hemisulfate | Erythromycin thiocyanate | Methyl 2-amino-5-bromobenzoate | Sofosbuvir | Guanidine hydrochloride | Hexane-1,6-diol | 1,4-Naphthoquinone | Adenine | Carbazole | Thymidine | Usnic Acid |
| Related Compound Libraries | DNA Damage & Repair Compound Library | Bioactive Compound Library | ReFRAME Related Library | Anti-Viral Compound Library | Drug Repurposing Compound Library | Inhibitor Library | Clinical Compound Library | Bioactive Compounds Library Max | Anti-Infection Compound Library | Nucleotide Compound Library |
United States
