| Name | RG13022 |
| Description | RG13022 (Tyrphostin RG13022) is a tyrosine kinase inhibitor; inhibits the autophosphorylation reaction of the EGF receptor (IC50: 4 μM). |
| Cell Research | 40 mM stock solutions of RG13022 is made in 100% DMSO and diluted with the culture medium before addition to the cells. MH-85 cells and HER 14 cells are plated in culture medium in the presence or absence of increasing concentrations of RG-13022 or RG-14620 for 10 days. At the end of culture, the cells are fixed with 4% (v/v) formaldehyde and stained with hematoxylin. Numbers of colonies including more than 20 cells in each well are counted under the microscope[1]. |
| In vitro | RG13022 suppresses EGF-stimulated cancer cell proliferation and inhibits EGF receptor autophosphorylation in immunoprecipitates (IC50: 4 μM) in a cell-free reaction. It inhibits colony formation (IC50: 1 μM) and DNA synthesis (IC50: 3 μM) in HER 14 cells stimulated by 50 ng/mL EGF, dose-dependently. Additionally, RG13022 inhibits growth stimulated by insulin, insulin-like growth factor I, insulin-like growth factor II, or transforming growth factor alpha. It completely blocks estrogen-stimulated phosphorylation of the EGF receptor and estrogen-induced cell proliferation, indicating that functioning TK pathways are essential for estrogen action. |
| In vivo | RG13022 suppresses tumor growth and extends the lifespan of tumor-bearing nude mice. |
| Storage | Shipping with blue ice/Shipping at ambient temperature. |
| Solubility Information | DMSO : 50 mg/mL (187.77 mM), Sonication is recommended.
10% DMSO+40% PEG300+5% Tween 80+45% Saline : 2 mg/mL (7.51 mM), Sonication is recommended.
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| Keywords | Tyrphostin RG-13022 | Tyrphostin RG 13022 | RG-13022 | RG13022 | Inhibitor | inhibit | HER1 | ErbB-1 | Epidermal growth factor receptor | EGFR |
| Inhibitors Related | (S)-Afatinib | Osimertinib | Lidocaine Hydrochloride hydrate | Lapatinib | Erlotinib hydrochloride | Erlotinib | Neratinib | Afatinib | Chalcone | Brigatinib | Genistein | Gefitinib |
| Related Compound Libraries | Anti-Colorectal Cancer Compound Library | Anti-Lung Cancer Compound Library | Bioactive Compound Library | Membrane Protein-targeted Compound Library | Kinase Inhibitor Library | Tyrosine Kinase Inhibitor Library | Anti-Ovarian Cancer Compound Library | JAK-STAT Compound Library | Inhibitor Library | Immunology/Inflammation Compound Library | Bioactive Compounds Library Max | Anti-Cancer Active Compound Library |
United States
