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ChemicalBook Product Catalog Chemical Reagents Organic reagents Azo, diazo compound Rhodamine 123

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Rhodamine 123 NEW

$47.00 5mg

$61.00 10mg

$82.00 25mg

Min. Order
Purity99.95%
Cas No62669-70-9
Supply Ability10g
Update time2026-04-13

TargetMol Chemicals Inc.

TargetMol Chemicals Inc.

VIP6Y

United States

United States

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Chemical Properties

Product Name	Rhodamine 123
CAS No	62669-70-9
EC-No
Min. Order
Purity	99.95%
Supply Ability	10g
Release date	2026/04/13

Product Introduction

Bioactivity

Name	Rhodamine 123
Description	Rhodamine 123 is a fluorescent dye (λex=503 nm, λem=527 nm).
Cell Research	Instructions I. Solution preparation 1. Preparation of mother solution: Dissolve Rhodamine 123 in anhydrous DMSO or ethanol to prepare a 1-5 mM stock solution. Note: Avoid vigorous stirring or shaking during dissolution to prevent fluorescence quenching. 2. Working solution preparation: Dilute the stock solution with appropriate culture medium to the required working concentration, usually 1-2 μM. II. Operation steps 1. Cell preparation: 1) Culture cells on a slide, the cell density should be 5×10^4 to 5×10^5 cells/mL. 2) Wash cells with PBS or Hank’s solution to remove residual culture medium. 2. Staining: Add the working concentration solution to the cells and incubate at 37°C in the dark for 30 minutes to 1 hour. 3. Washing: 1) Remove the dye solution and wash the cells with culture medium or PBS to remove unbound dye. 2) If cells need to be fixed, add 10% formalin buffer and incubate for 15-20 minutes, then wash with PBS. 4. Observation: Use a fluorescence microscope with a fluorescein filter to observe cells. Rhodamine 123 is excited at a wavelength of about 507 nm and emits green fluorescence at 529 nm. Precautions: 1) Wear gloves during operation to avoid contact between skin or mucous membranes and reagents. 2) Avoid light during incubation and storage to prevent fluorescence quenching. 3) After staining, fluorescence detection analysis should be performed immediately. The above information is based on published literature. Experimental procedures should be appropriately modified to meet specific research demands.
In vitro	The intensity of R123 fluorescence has a peak at a concentration of 50 μM and decreases to zero at higher concentrations because of self-quenching[1].
Storage	Shipping with blue ice/Shipping at ambient temperature.
Solubility Information	DMSO : 30 mg/mL (78.78 mM), Sonication is recommended.
Keywords	Rhodamine-123 \| Rhodamine123 \| Rhodamine 123 \| RH123 \| RH 123 \| R22420 \| R 22420 \| Inhibitor \| inhibit
Related Compound Libraries	ReFRAME Related Library

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