Rhodamine 800
 Rhodamine 800 structure | Common Name | Rhodamine 800 |
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| CAS Number | 137993-41-0 | Molecular Weight | 495.955 |
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| Density | N/A | Boiling Point | N/A |
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| Molecular Formula | C26H26ClN3O5 | Melting Point | N/A |
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| MSDS | USA | Flash Point | N/A |
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Use of Rhodamine 800 Rhodamine 800 is a near-infrared fluorescent dye. |
Properties
Articles4
Spectrum
Names
| Name | Rhodamine 800 |
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| Synonym | More Synonyms |
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Rhodamine 800 Biological Activity
| Description | Rhodamine 800 is a near-infrared fluorescent dye. |
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| Related Catalog | Signaling Pathways >> Others >> Others Dye Reagents Research Areas >> Others |
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| In Vitro | Rhodamine 800 has different optical absorption spectra in various organic solvents and in aqueous solution in the visible and near-infrared regions. The absorption maximum at 683 nm in acetone shifts to 696 nm in DMSO without a significant change of the overall spectral profile. The fluorescence intensity of Rhodamine 800 varies markedly with the solvent, and decreases to less than one-fifth when the solvent is changed from acetone to water without a significant change of the emission peak[1]. |
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| Kinase Assay | Rhodamine 800 (Rh800) and indocyanine green (ICG) are dissolved in PBS and their concentrations are set to 40 nM. To 3 mL of Rhodamine 800 solution (40 nM/PBS), 100 μL of p-sulfonatocalix[n]arenes (S[n]) (1 mM) is added. To this solution, microliter aliquots of acetylcholine (ACh) (350 mM) are added under stirring. After 2 min stirring, fluorescence spectra are measured. For the competitive fluorophore displacement by other neurotransmitters, amino acids, ammonium chloride, and choline, similar procedure as the above method is applied[2]. |
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| References | [1]. Sakanoue J, et al. Rhodamine 800 as a probe of energization of cells and tissues in the near-infrared region: a study with isolated rat liver mitochondria and hepatocytes. J Biochem. 1997 Jan;121(1):29-37. [2]. Jin T. et al. Near-infrared fluorescence detection of acetylcholine in aqueous solution using a complex ofrhodamine 800 and p-sulfonatocalix[8]arene. Sensors (Basel). 2010;10(3):2438-49. |
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Chemical & Physical Properties
| Molecular Formula | C26H26ClN3O5 |
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| Molecular Weight | 495.955 |
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| Exact Mass | 495.156097 |
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