| Name | SMCC |
| Description | SMCC (N-Succinimidyl 4-(N-maleimidomethyl)cycl) is a heterobifunctional protein crosslinker. |
| Cell Research | Branched PEI (25 kDa) was dissolved in PBS buffer to yield a concentration of 1 mg/mL. No-weigh Sulfo-SMCC was dissolved in Milli-Q water with a concentration of 10 mg/mL, then a certain of the prepared no-weigh SulfoSMCC solution was added into 2 mL PEI solution to incubate for 30 min at 37 C. After the PEI-SMCC polymer was formed, TAT solution was added into the polymer at the given molar ratio and incubated for 2 h at 4 C. The concentration of unreacted SMCC and unreacted TAT were removed using Zeba Spin Desalting Columns. The polymer PEI-SMCC-TAT was also characterized and analyzed through FTIR to check if each element was added to the PEI backbone. |
| In vitro | N-Succinimidyl 4-(N-maleimidomethyl)cycl（SMCC) is an amine-to-sulfhydryl crosslinker that contains NHS-ester and maleimide reactive groups at opposite ends of a medium-length cyclohexane-stabilized spacer arm (8.3 angstroms). SMCC conjugation occurs via a maleimide group that is sulfhydryl (thiol; -SH) reactive and a NHS ester group that is amine reactive and forms stable, covalent protein crosslinks. |
| Storage | store at low temperature,keep away from direct sunlight,store under nitrogen | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature. |
| Solubility Information | DMSO : 72.14 mg/mL (215.78 mM), Sonication is recommended.
10% DMSO+40% PEG300+5% Tween 80+45% Saline : 2 mg/mL (5.98 mM), Sonication is recommended.
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| Keywords | SMCC | Inhibitor | inhibit | Antibody-drug conjugates linkers | ADCLinker | ADC Linkers | ADC Linker |
| Inhibitors Related | N-Boc-PEG5-bromide | Boc-C16-COOH | BMPS | 4-Azidobenzyl alcohol | Succinic anhydride | DSS Crosslinker | H-Glu-OtBu | Fmoc-Gly-Gly-OH | Fmoc-8-amino-3,6-dioxaoctanoic acid | N-Hydroxysulfosuccinimide sodium | EMCS | DSG Crosslinker |
| Related Compound Libraries | NO PAINS Compound Library | Immunology/Inflammation Compound Library |
United States
