| Name | Spermidine |
| Description | Spermidine (N-(3-Aminopropyl)-1,4-diaminobutane) inhibits NOS1 (nNOS). Spermidine binds and precipitates DNA and may be used for purification of DNA binding proteins. Spermidine activates PNK (polynucleotide kinase T4). Spermidine binds to and activates NMDA and has been shown to potentiate NMDA-induced currents in a concentration-dependent manner. |
| In vitro | METHODS: Human neuroblastoma cells SH-SY5Y were treated with Spermidine (0.05-10 µM) for 24-48 h. Mitochondrial metabolic activity was measured by MTT assay and ATP production assay.
RESULTS: No effect of Spermidine was observed after 24 h. 0.1 and 1 µM Spermidine significantly increased cellular metabolic activity and ATP production after 48 h of treatment. The most effective concentration of Spermidine, 0.1 µM, induced an 8.2% increase in metabolic activity and a 4% increase in ATP production. [1]
METHODS: The retinal pigment epithelial cell line ARPE-19 was treated with H2O2 (300 µM) and Spermidine (10 µM) for 24 h. Apoptosis was detected by Flow cytometry.
RESULTS: 300 µM H2O2 significantly increased the frequency of annexin V-positive cells to approximately 25%, but Spermidine significantly inhibited this increase. The results suggest that Spermidine attenuates H2O2-mediated oxidative stress-induced apoptosis in ARPE-19 cells. [2] |
| In vivo | METHODS: To study the effects on diabetes, Spermidine (10 mM) was administered orally in water to non-obese diabetic (NOD) mice (type 1 diabetes mouse model) for thirty weeks.
RESULTS: Treatment of NOD mice with Spermidine resulted in a higher incidence of diabetes, although pancreatic insulitis was not altered.Spermidine modulated pancreatic tissue polyamine levels and increased signs of autophagy.Spermidine resulted in an increase in the proportion of pro-inflammatory T-cells in the pancreatic lymph nodes (pLN) of diabetic mice. [3] |
| Storage | Pure form: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature. |
| Solubility Information | 5% DMSO+95% Saline : 1.67 mg/mL (11.5 mM), Solution.
DMSO : 250 mg/mL (1721.17 mM), Sonication is recommended.
H2O : 100 mg/mL (688.47 mM), Sonication is recommended.
|
| Keywords | stability | Spermidine | PSII | photosystem | membrane | Inhibitor | inhibit | Human Endogenous Metabolite | H2O2 | EndogenousMetabolite | Endogenous Metabolite | cell |
| Inhibitors Related | Sucrose | Aceglutamide | Nicotinamide riboside malate | DL-Lysine | D(+)-Raffinose pentahydrate | Guanidine hydrochloride | Formamide | Glycerol | Thymidine | Corn starch | Hyaluronic acid sodium (MW 20 kDa) | Gluconate Calcium |
| Related Compound Libraries | Bioactive Compound Library | Traditional Chinese Medicine Monomer Library | Selected Plant-Sourced Compound Library | Natural Product Library | Drug Repurposing Compound Library | Anti-Inflammatory Traditional Chinese Medicine Compound Library | Natural Product Library for HTS | Cosmetic Ingredient Compound Library | Immunology/Inflammation Compound Library | Anti-Aging Compound Library | Bioactive Compounds Library Max | Heat-Clearing and Detoxifying Traditional Chinese Medicine Compound Library |
United States
