| Name | SR-3677 |
| Description | SR-3677 is an effective and specific inhibitor of ROCK2 (IC50: 3 nM). |
| Kinase Assay | 5 μL mixture of a 1 μM STK2 substrate and ATP (ROCK-I: 4 μM; ROCK-II: 20 μM) in STK-buffer is added to the wells. 20 nL of test compounds (SR-3677) is dispensed. The reaction is started by addition of 5 μL of 2.5 nM. ROCK-I or 0.5 nM ROCK-II in STK-buffer. After 4 h at RT, the reaction is stopped by the addition of 10 μL of 1x antibody and 62.5 nM Sa-XL in the detection buffer. |
| In vitro | SR-3677 has an IC50 of ~3 nM in enzyme and cell-based assays and has an off-target hit rate of 1.4% against 353 kinases, and inhibits only 3 out of 70 non-kinase enzymes and receptors. The IC50 value of SR-3677 for ROCK-I is 56 nM. |
| In vivo | SR-3677 increases ex vivo aqueous humor outflow in porcine eyes and inhibiting myosin light chain phosphorylation. Continuous exposure of 25 μM SR-3677 increases the outflow facility by 60% at 1 h perfusion, increasing to 70–80% for the 2–5 h time points. |
| Storage | Shipping with blue ice/Shipping at ambient temperature. |
| Solubility Information | 10% DMSO+40% PEG300+5% Tween 80+45% Saline : 2 mg/mL (4.9 mM), Sonication is recommended. DMSO : 40 mg/mL (97.93 mM), Sonication is recommended.
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| Keywords | SR-3677 | SR3677 | SR 3677 | ROK | ROCK2 | ROCK1 | ROCK | Rho-kinase | Rho-associated protein kinase | Rho-associated kinase | Inhibitor | inhibit | Autophagy |
| Inhibitors Related | Stavudine | Aceglutamide | Hemin | Tamoxifen | Cysteamine hydrochloride | Guanidine hydrochloride | Hydroxychloroquine | Enzalutamide | Paeonol | Naringin | Alginic acid | Sildenafil citrate |
| Related Compound Libraries | Reprogramming Compound Library | Neural Regeneration Compound Library | Bioactive Compound Library | Membrane Protein-targeted Compound Library | Kinase Inhibitor Library | Inhibitor Library | Stem Cell Differentiation Compound Library | NO PAINS Compound Library | Bioactive Compounds Library Max | Wnt/Hedgehog/Notch Compound Library | Anti-Cancer Compound Library | TGF-beta/Smad Compound Library |