| Name | Talacotuzumab |
| Description | Talacotuzumab (JNJ 56022473) is an IgG1 fully humanized CD123 neutralizing monoclonal antibody containing a modified Fc structure. Talacotuzumab has a high affinity for CD123, CD32b/c, CD16-158F and CD16-158V, with KD of 0.43 nM, 188 nM, 46 nM and 16.8 nM, respectively. Talacotuzumab inhibits IL-3 signaling in target cells by inhibiting IL-3 binding to CD123. Talacotuzumab induces mutations in the Fc region to increase affinity for CD16 (FCγriiia), thereby enhancing antibody-dependent cell-mediated cytotoxicity (ADCC). Talacotuzumab inhibits leukemia cell growth in xenografted mouse models of acute myeloid leukemia (AML). |
| In vitro | With an IC50 of 5 ng/ml (33 pM), Talacotuzumab (JNJ 56022473; CSL 362) potently mediates antibody-dependent cell-mediated cytotoxicity (ADCC) in TF-1 cells [1].
Following a 24-hour pretreatment at 1 μg/ml,Talacotuzumab inhibits the production of IFN-α in plasmacytoid dendritic cells and eosinophils in SLE (systemic lupus erythematosus) donor and healthy donor plasma-like dendritic cells. This inhibition occurs when these cells are stimulated with TLR7 (imiquimod; 0.5 μM; 6 days) and TLR9 (CpG C; 0.5 μM; 6 days). However, Talacotuzumab does not significantly reduce IFN-α production induced by TLR4 stimulation (LPS; 10 μg/ml). The inhibitory effect of Talacotuzumab on plasmacytoid dendritic cell (pDC) proliferation and expansion induced by TLR7 and TLR9 is achieved through the depletion of pDC [2]. |
| In vivo | In mice with xenografts of acute myeloid leukemia,Talacotuzumab (JNJ 56022473; CSL 362; 300 μg; intraperitoneal injection; three times per week for 5 weeks) significantly delays tumor growth compared to the isotype control [1].
Talacotuzumab (1, 10, 30 mg/kg; subcutaneous injection; single dose) in juvenile cynomolgus monkeys reaches maximum serum concentrations of 12, 190, and 380 μg/ml at doses of 1, 10, and 30 mg/kg, respectively, within 48 hours [2]. |
| Storage | Shipping with blue ice/Shipping at ambient temperature. |
| Keywords | Talacotuzumab | JNJ-56022473 | JNJ56022473 | Interleukin Related | Inflammationrelated | Inflammation related | Immunologyrelated | Immunology related | CSL-362 | CSL362 | CD123 |
| Inhibitors Related | Kaolin | Undecane | Fluorene | Ethyl palmitate | Rhamnose monohydrate | Isostearic acid | D(+)-Raffinose pentahydrate | Hydroxypropyl Cellulose | 1,8-Cineole | Tributyrin | Gluconate Calcium | Magnesium sulfate |
United States
