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ChemicalBook Product Catalog Biochemical Engineering Biochemical Reagents Biological Dyes TMB-PS

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TMB-PS NEW

$33.00 50mg

$48.00 100mg

$68.00 200mg

Min. Order
Purity≥95%
Cas No102062-36-2
Supply Ability10g
Update time2026-04-13

TargetMol Chemicals Inc.

TargetMol Chemicals Inc.

VIP6Y

United States

United States

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Chemical Properties

Product Name	TMB-PS
CAS No	102062-36-2
EC-No
Min. Order
Purity	≥95%
Supply Ability	10g
Release date	2026/04/13

Product Introduction

Bioactivity

Name	TMB-PS
Description	TMB-PS (TMBZ-PS) is a chromogenic reagent for the detection of hydrogen peroxide e.g. in the enzymatic analysis of body fluids.
Cell Research	Instructions I. Solution preparation 1. Stock solution: Dissolve TMB-PS in deionized water or an appropriate buffer, usually 1–10 mM stock solution. 2. Working solution: Dilute the stock solution to a working concentration, usually 0.1–1 mM, according to the experimental requirements. To enhance the reaction rate and sensitivity, it is recommended to use a solution containing hydrogen peroxide as the substrate solution. II. Operation steps 1. Enzyme-linked immunosorbent assay (ELISA): (1) Add specific antibodies or antigens to the wells of the ELISA plate and incubate. (2) Add an enzyme marker that binds to the target antibody or antigen. (3) Wash the plate with an appropriate buffer. (4) Add TMB-PS substrate solution (usually containing hydrogen peroxide) and incubate in the dark for a certain period of time (5–30 minutes) until a blue or purple reaction product is observed. (5) Stop reaction: Add an acidic solution (such as 2 M sulfuric acid or 1 M phosphoric acid) to terminate the reaction. The reactant changes from blue to yellow, which facilitates the reading of optical density. 2. Immunohistochemical staining: (1) Add TMB-PS substrate solution to tissue sections or cell cultures for staining. (2) Wash off excess dye with PBS and observe under a microscope. 3. Calibration and control: (1) Control group: Set up a group without substrate solution as a control to ensure the specificity of the substrate reaction. (2) Standard curve: A standard curve between optical density and concentration can be established using samples of known concentration for quantitative analysis. Notes: (1) Photosensitivity: TMB-PS is light-sensitive and should be avoided from prolonged exposure to strong light. (2) Storage conditions: TMB-PS should be stored in a dry, cool place, away from high temperatures and strong light, usually at -20°C. (3) pH range: TMB-PS performs best in an acidic environment of pH 3–5, but is also stable over a wider pH range. (4) Solubility: TMB-PS has good solubility in water, but it should be ensured that there are no undissolved solid particles. The above information is based on published literature. Experimental procedures should be appropriately modified to meet specific research demands.
Storage	Keep away from direct sunlight \| Shipping with blue ice/Shipping at ambient temperature.
Solubility Information	DMSO : 35 mg/mL (96.55 mM), Sonication is recommended.
Keywords	TMB-PS \| TMBPS \| TMB PS \| Inhibitor \| inhibit
Related Compound Libraries	Bioactive Compound Library \| NO PAINS Compound Library \| Bioactive Compounds Library Max \| Anti-COVID-19 Compound Library

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