TRIS hydrochloride

Tris HCl was used to grow protein crystals at different pH. The low ionic strength of Tris HCL buffer can be used to form intermediate fibers of lamin in C. elegans. Tris HCl is also one of the main components of protein electrophoresis buffer. In addition, Tris HCL is also an intermediate for the preparation of surfactants, vulcanization accelerators and some drugs. Tris HCl is also used as a titration standard. The preparation of buffer solution The Tris HCl buffer solution (Tris HCl buffer solution) with a concentration of 6.057g/L and pH=8.8 was prepared by adjusting the pH value of HCl aqueous solution with a concentration of 0.1mol/L, using trihydroxymethyl aminomethane hydrochloride as the solute and deionized water as the solvent. Chemical property White crystal. Purpose The pH value of all buffer solutions depends on temperature and concentration. For Tris buffer, the pH increases by about 0.03 units for each 1 ° C increase in temperature and decreases by 0.03-0.05 units for every 10 times the diluted pH. Uses: used as biological buffer, used as organic synthesis intermediate