Human TSH alpha/beta Heterodimer Protein, His Tag
Synonym
TSH alpha, beta Heterodimer, TSH, Thyrotropin
Source
Human TSH alpha/beta Heterodimer, His Tag (TSR-H52W8) is expressed from human 293 cells (HEK293). It contains AA Phe 21 - Val 138 (TSH beta) & Ala 25 - Ser 116 (TSH alpha) (Accession # P01215-1 (TSH alpha) & P01222-1 (TSH beta)).
Predicted N-terminus: Phe 21
Molecular Characterization
This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 28.4 kDa. The protein migrates as 43-48 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 1.0 EU per μg by the LAL method / rFC method.
Purity
>90% as determined by SDS-PAGE.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
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Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.
Background
Thyroid-stimulating hormone (also known as thyrotropin, thyrotropic hormone, or abbreviated TSH) is a pituitary hormone that stimulates. It is a glycoprotein hormone produced by thyrotrope cells in the anterior pituitary gland, which regulates the endocrine function of the thyroid. Thyroid stimulating hormone consists of two subunits called alpha and beta. The alpha and beta subunits are bound together to produce the active form of the hormone. The α subunit is thought to be the effector region responsible for stimulation of adenylate cyclase (involved the generation of cAMP).The β (beta) subunit (TSHB) is unique to TSH, and therefore determines its receptor specificity.
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