| Name | WS6 |
| Description | WS6, a β cell proliferation inducer, regulates Erb3 binding protein-1 (EBP1) and the IκB kinase pathway. |
| Cell Research | R7T1 cells are growth-arrested by removal of doxycycline for 2 days and plated into 384-well plates at a density of 3,000 cells/well in growth medium. Β cell proliferation is assessed using CellTiter Glo after 4 days. Fold increase in cell number is calculated by normalizing compound-treated wells to the median of DMSO-treated wells. (Only for Reference) |
| Kinase Assay | PARP assays are conducted in a buffer containing 50 mM Tris (pH 8.0), 1 mM DTT, 1.5 μM [3H]NAD+?(1.6 μCi/mmol), 200 nM biotinylated histone H1, 200 nM slDNA, and 1 nM PARP-1 or 4 nM PARP-2 enzyme. Reactions are terminated with 1.5 mM benzamide, transferred to streptavidin Flash plates, and counted using a TopCount microplate scintillation counter. |
| In vitro | In the RIP-DTA mouse model with β-cell ablation, oral administration of WS6 (50 mg/kg) promotes β-cell proliferation, thereby ameliorating diabetes. |
| In vivo | In R7T1 cells (EC50=0.28 μM), WS6 induces cell proliferation through the action on the Erb3 binding protein-1 and IκB kinase signaling pathways. |
| Storage | 02 | Shipping with blue ice/Shipping at ambient temperature. |
| Solubility Information | 10% DMSO+40% PEG300+5% Tween 80+45% Saline : 3.3 mg/mL (5.8 mM), Sonication is recommended.
H2O : < 1 mg/mL (insoluble or slightly soluble)
DMSO : 93 mg/mL (163.56 mM), Sonication is recommended.
Ethanol : 93 mg/mL (163.56 mM), Sonication is recommended.
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| Keywords | WS-6 | WS6 | WS 6 | IκB kinase | IκB | Inhibitor | inhibit | IKK | IkB/IKK | IkB | ErbB3-Binding Protein-1 (EBP2) |
| Inhibitors Related | Musk ketone | Myricetin | Creatine monohydrate | p-Hydroxybenzaldehyde | Hyaluronic acid | Urethane | Sorafenib | Penicillin G sodium salt | Valproic Acid | Methyl eugenol | Gefitinib | Dihydromyricetin |
| Related Compound Libraries | Anti-Lung Cancer Compound Library | NF-κB Signaling Compound Library | Reprogramming Compound Library | Bioactive Compound Library | Membrane Protein-targeted Compound Library | Tyrosine Kinase Inhibitor Library | Kinase Inhibitor Library | NO PAINS Compound Library | Anti-Aging Compound Library | Immunology/Inflammation Compound Library | Bioactive Compounds Library Max | Fluorochemical Library |
United States
