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ChemicalBook Product Catalog Biochemical Engineering Saccharides X-GAL

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X-GAL NEW

$ 56.00 /500mg

Min. Order
Purity99.42%
Cas No7240-90-6
Supply Ability10g
Update time2026-04-13

TargetMol Chemicals Inc.

TargetMol Chemicals Inc.

VIP6Y

United States

United States

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Chemical Properties

Product Name	X-GAL
CAS No	7240-90-6
EC-No
Min. Order
Purity	99.42%
Supply Ability	10g
Release date	2026/04/13

Product Introduction

Bioactivity

Name	X-GAL
Description	X-GAL (BCIG) is a widely used chromogenic substrate for β-galactosidase, which cleaves X-GAL to produce an insoluble blue compound.
In vitro	Instructions: I. Solution preparation Mother solution preparation: Prepare X-GAL stock solution: Dissolve X-GAL in anhydrous dimethylformamide (DMF) or dimethyl sulfoxide (DMSO) to prepare a 20 mg/mL stock solution. Note: Protect from light, dispense in small volumes (e.g. 50–100 μL), and store at -20°C. The storage time should not be too long to avoid degradation. II. Operation steps 1. Prepare color reaction mixture 1) For plate screening: Add X-GAL and IPTG (isopropyl-β-D-thiogalactoside) to LB agar plates. Common final concentrations: X-GAL 40 µg/mL, IPTG 0.1 mM. Add X-GAL and IPTG when the agar medium cools to about 50°C, mix well, and pour onto the plate. 2). For liquid color development: Add X-GAL and IPTG to the reaction buffer, the usual concentration is the same as for plate screening. 2. Experimental application Blue-white screening: 1) Transform the vector containing the reporter gene (such as lacZ or lacZα) into the host bacteria (such as E. coli DH5α). 2) Spread on a plate containing X-GAL and IPTG and culture at 37°C for 12–16 hours. 3) Screening results: Blue colonies indicate positive β-gal activity (lacZ expression), and white colonies indicate negative (lacZ is not expressed). Tissue staining: 1) After appropriate tissue fixation and permeabilization, use a reaction buffer containing X-GAL (usually PBS at pH 7.2–7.5, containing Mg²⁺). 2) Incubate at 37°C for 12–24 hours, and observe the blue precipitate under a microscope for color development. The above information is based on published literature. Experimental procedures should be appropriately modified to meet specific research demands.
Storage	Shipping with blue ice/Shipping at ambient temperature.
Solubility Information	10% DMSO+40% PEG300+5% Tween-80+45% Saline : 3.3 mg/mL (8.08 mM), Sonication is recommended. DMSO : 147 mg/mL (359.74 mM), Sonication is recommended.
Keywords	X-GAL \| XGAL \| X GAL \| Inhibitor \| inhibit
Inhibitors Related	Cedryl acetate \| Hexylresorcinol \| Conduritol B epoxide \| Gcase activator 2 \| Fucoidan \| Miglitol \| Ambroxol hydrochloride \| D-(-)-Quinic acid \| (E)-3-(4-Methoxyphenyl)acrylic acid \| Naringinase \| Acarbose \| 3-Butylidenephthalide
Related Compound Libraries	Bioactive Compound Library \| Bioactive Compounds Library Max

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