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ChemicalBook Product Catalog Biochemical Engineering Saccharides Monosaccharide X-Gluc Dicyclohexylamine

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X-Gluc Dicyclohexylamine NEW

$30.00 2mg

$42.00 5mg

$61.00 10mg

Min. Order
Purity99.15%
Cas No18656-96-7
Supply Ability10g
Update time2026-04-13

TargetMol Chemicals Inc.

TargetMol Chemicals Inc.

VIP4Y

United States

United States

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Chemical Properties

Product Name	X-Gluc Dicyclohexylamine
CAS No	18656-96-7
EC-No
Min. Order
Purity	99.15%
Supply Ability	10g
Release date	2026/04/13

Product Introduction

Bioactivity

Name	X-Gluc Dicyclohexylamine
Description	X-Gluc Dicyclohexylamine (5-Bromo-4-chloro-3-indolyl-beta-D-glucuronide cyclohexylammonium salt) is a reagent to detect β-glucuronidase and can be used in molecular biology experiments to mark and select the expression of target genes.
Cell Research	Instructions I. Solution preparation 1. Stock solution: Dissolve X-Gluc Dicyclohexylamine powder in DMSO (dimethylsulfonamide) or acetone, the stock solution concentration is usually 50-100 mg/mL. 2. Working solution: Dilute the stock solution to a final concentration of 1-2 mg/mL using sodium phosphate buffer (pH 7.0), but the optimal concentration may need to be optimized depending on the application. II. Histochemical staining procedure 1. Sample preparation: Prepare plant tissues, cells or other samples to be stained. For plant tissues, samples may need to be fixed and permeabilized with a fixative such as formalin or glutaraldehyde. 2. Incubation: Incubate the prepared sample with X-Gluc working solution (1-2 mg/mL), usually at 37°C for several hours (usually 4-16 hours). The incubation time can be adjusted according to the sensitivity of the sample and the intensity of the desired staining. 3. Visualization: After the incubation period, wash the sample to remove excess substrate and it can then be observed under an optical microscope. β-Glucuronidase activity will result in the formation of an indigo-colored precipitate, resulting in a blue stain in the tissue. 4. Counterstain (optional): In some cases, a counterstain such as eosin may be used to highlight other features of the tissue or cells. The above information is based on published literature. Experimental procedures should be appropriately modified to meet specific research demands.
Storage	Shipping with blue ice/Shipping at ambient temperature.
Solubility Information	DMSO : 30 mg/mL (49.67 mM), Sonication is recommended. 10% DMSO+40% PEG300+5% Tween-80+45% Saline : 2 mg/mL (3.31 mM), Sonication is recommended.
Keywords	X-Gluc Dicyclohexylamine \| XGluc Dicyclohexylamine \| X-Gluc \| X Gluc Dicyclohexylamine \| Inhibitor \| inhibit

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