| Name | Z-DEVD-FMK |
| Description | Z-DEVD-FMK (Caspase-3 Inhibitor) is a Caspase-3 inhibitor (IC50=18 μM), featuring selectivity, irreversibility and cell permeability. Z-DEVD-FMK can inhibit caspase activation induced apoptosis. |
| Cell Research | N27 cells are incubated with 100 μM 6-OHDA for 24 h or 300 μM MPP+ for 36 h in the presence or absence of 50 μM Z-DEVD-FMK and cell death is determined by MTT (3-(4,5-dimethylthiazol-3-yl)-2,5-diphenyl tetrazolium bromide) assay, which is widely used to assess cell viability. After treatment, the cells are incubated in serum-free medium containing 0.25 mg/ml MTT for 3 h at 37°C. Formation of formazan from tetrazolium is measured at 570 nm with a reference wavelength at 630 nm using a SpectraMax microplate reader.(Only for Reference) |
| Kinase Assay | Caspase activity assay : Caspase-3 and caspase-9 activities are measured using fluorescent-based substrate. After treatment, the cells are resuspended in lysis buffer (50 mM Tris HCl, 1 mM EDTA, and 10 mM EGTA) containing 10 mM digitonin for 20 min at 37°C. Supernatants are treated with either of the fluorogenic substrates Ac-DEVD-AFC for caspase-3 or Ac-LEHD-AFC for caspase-9 for 1 h at 37°C and fluorescence is measured at excitation at 400 nm and emission at 505 nm using a Gemini XS fluorescence plate reade |
| In vitro | METHODS: v-K-ras transformed normal rat kidney cells KNRK were treated with SCH56582 (20 µM) and Z-DEVD-FMK (20-50 µM) for 24 h. Cell death was detected using trypan blue.
RESULTS: Addition of 50 µM Z-DEVD-fmk resulted in >70% inhibition of apoptosis. [1]
METHODS: Human multiple myeloma cells KM3 were treated with betulinic acid (15 µg/mL) and Z-DEVD-FMK (50 mol/L) for 24 h, and the expression level of target proteins was detected using Western Blot.
RESULTS: Z-DEVD-FMK attenuated the betulinic acid-induced activation of caspase 3. Z-DEVD-FMK significantly blocked the cleavage of PARP. Z-DEVD-FMK significantly blocked PARP cleavage. [2] |
| In vivo | METHODS: To test the in vivo activity, Z-DEVD-FMK (1.8 mg/kg in ethanol and freshly diluted in PBS containing 2% Tween-80) was injected intraperitoneally into C57BL/6 mice, followed by CPT-11 (350 mg/kg) 30 min later. Z-DEVD-FMK was continued to be administered once daily for three days.
RESULTS: Caspase-3 inhibitor Z-DEVD-FMK attenuated PT-11-induced GATA6-deficient mouse peritoneal macrophages. [3] |
| Storage | store under nitrogen,store at low temperature | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature. |
| Solubility Information | H2O : < 1 mg/mL (insoluble or slightly soluble)
10% DMSO+40% PEG300+5% Tween 80+45% Saline : 5 mg/mL (7.48 mM), Sonication is recommended.
DMSO : 252.5 mg/mL (377.62 mM), Sonication is recommended.
Ethanol : < 1 mg/mL (insoluble or slightly soluble)
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| Keywords | Z-DEVD-FMK | ZDEVDFMK | Z DEVD FMK | Inhibitor | inhibit | Caspase-3 | Caspase |
| Inhibitors Related | 10-Hydroxydecanoic Acid | Ascorbyl palmitate | Pendimethalin | Hydralazine hydrochloride | N-Hydroxyphthalimide | Berberine hydrogen sulphate | Cystamine dihydrochloride | Copper Sulfate Pentahydrate | (Iso)-Z-VAD(OMe)-FMK | Tauroursodeoxycholate | Berberine sulfate | Cinchonine |
| Related Compound Libraries | Highly Selective Inhibitor Library | Apoptosis Compound Library | Bioactive Compound Library | Anti-Neurodegenerative Disease Compound Library | Membrane Protein-targeted Compound Library | Pyroptosis Compound Library | Peptide Compound Library | Inhibitor Library | Anti-Aging Compound Library | Bioactive Compounds Library Max | Fluorochemical Library | Human Metabolite Library |
United States
