| Name | ZMC1 |
| Description | NSC-319726 (ZMC1), a mutant p53R175 reactivator, suppresses growth of fibroblasts that expresses the p53R175 mutation (IC50 = 8 nM). |
| Cell Research | Viability assays are done. The cells (5×104 cells/well, in 1 ml culture) are cultured in a 12-well plate to reach the 50%–60% confluence on the second day when treated with serial dilutions of the compound. The growth is measured by Guava ViaCount reagent and Guava PCA instrument after incubation for 3 days.(Only for Reference) |
| Kinase Assay | MTS: 5,000 cells of TOV112D cells (5,000 cells/well, in 100 ml culture) are cultured in 96-well plate to reach the 50%–60% confluence on the second day when treated with serial dilutions of the compounds. The growth is measured by MTS reagent and Victor Plate reader instrument after incubation for 3 days. |
| In vitro | In cells with mutant p53, NSC319726 (IC50=8 nM) causes a WT-like conformational change in the mutant protein that repairs sequence-specific p53 transcription. |
| In vivo | In cells with mutant p53, NSC319726 (IC50=8 nM) causes a WT-like conformational change in the mutant protein that repairs sequence-specific p53 transcription. |
| Storage | Shipping with blue ice/Shipping at ambient temperature. |
| Solubility Information | 10% DMSO+40% PEG300+5% Tween 80+45% Saline : 2 mg/mL (8.54 mM), Sonication is recommended.
Ethanol : 2.3 mg/mL (9.82 mM), Heating is recommended.
DMSO : 23.4 mg/mL (99.86 mM), Sonication is recommended.
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| Keywords | ZMC-1 | ZMC 1 | p53 (R175) | NSC-319726 | NSC319726 | NSC 319726 | MDM-2/p53 | Inhibitor | inhibit |
| Inhibitors Related | 10-Hydroxydecanoic Acid | Flubendazole | Diuron | Gallium maltolate | Rotenone | D-(-)-3-Phosphoglyceric acid disodium | Triglycidyl isocyanurate | Indole-3-carbinol | Methyl methanesulfonate | (E/Z)-10-Hydroxy-2-decenoic acid | Magnolol | Diethanolamine hydrochloride |
| Related Compound Libraries | Apoptosis Compound Library | Anti-Pancreatic Cancer Compound Library | Bioactive Compound Library | HIF-1 Signaling Pathway Compound Library | Anti-Cancer Metabolism Compound Library | Glutamine Metabolism Compound Library | NO PAINS Compound Library | Metabolism Compound Library | Anti-Aging Compound Library | Bioactive Compounds Library Max | Anti-Cancer Compound Library | Transcription Factor-Targeted Compound Library |
United States
