文章标题：CHMP1A suppresses the growth of renal cell carcinoma cells via regulation of the PI3K/mTOR/p53 signaling pathway
作者列表：Wu Youping, Wu Yueguo, Xu Cong, Sun Wei, You Zhenqiang, Wang Yin, Zhang Sheng
期刊：Genes & Genomics
发表时间：2022-5-18
影响因子：2.164
DOI:10.1007/s13258-022-01237-w
主要研究成果：Abstract
Background
CHMP1A, a member of the ESCRT-III complex family, has been indicated as a brand-new inhibitor gene of tumors. Our previous research has revealed that CHMP1A plays a vital role in the development and progression of renal cell carcinoma (RCC).
Objective
To investigate the potential target pathway of the regulation of the tumor cell growth by CHMP1A.
Methods
The effect of CHMP1A on mTOR pathway was elucidated by western blotting. The effect of CHMP1A on the expression of p53 was evaluated, and A498 cell growth was assessed by colony formation and MTT assays. The expression of p53 was knocked down by shRNA-p53, and the effect of CHMP1A on mTOR after knockdown of p53 was evaluated. The effect of CHMP1A on apoptosis and its relationship with MDM2 pathway were detected by western blotting and FCM. Finally, the relationship between the regulation of p53 by CHMP1A and the PI3K/mTOR pathway was detected.
