ELIPA 驱动蛋白ATP酶检测试剂盒（酶联无机磷酸盐测定）

中文名称

ELIPA 驱动蛋白ATP酶检测试剂盒（酶联无机磷酸盐测定）

英文名字

Kinesin ELIPA (Enzyme Linked Inorganic Phosphate Assay TM)

供应商

Cytoskeleton

产品货号

BK060

产品报价

￥12484.368/KIT96assays

产品说明书

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购买方式

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产品新闻

背景资料

该ELIPA 驱动蛋白ATP酶检测试剂盒（酶联无机磷酸盐测定）产品是Cytoskeleton品牌原装进口，Cytoskeleton品牌是细胞骨架研究专家，专注于生物化学和细胞过程研究中的纯化蛋白和便捷试剂盒开发与生产。艾美捷作为中国区域的总代理，是行业中少有的致力于服务客户，帮助客户，且拥有独立专业技术支持团队的高科技生物企业。可以为您提供及时的咨询响应，专业的产品和解决方案支持，稳健快捷的交货周期，优质放心的售后服务。我们致力于为您提供有价值的产品和服务，在意您的成功！

产品描述

收到该ELIPA 驱动蛋白ATP酶检测试剂盒（酶联无机磷酸盐测定）Kinesin ELIPA (Enzyme Linked Inorganic Phosphate Assay TM)产品后，查看厂家提供的产品说明书详细介绍。

产品特点

Eukaryotic kinesin motor proteins orchestrate a wide range of kinetic events within a cell. They have been shown to move cargoes, such as chromosomes and vesicles, along microtubule tracks (1). They also play a major role in the organization of cytoskeletal architecture as evidenced in the establishment of the microtubule spindle during mitosis (2). Kinesins operate by utilizing the energy of ATP hydrolysis to move along their microtubule (MT) substrates. Once a kinesin motor binds to its MT track, the ATPase rate of the motor is often enhanced several hundred to several thousand fold (3). MT activated kinesin ATPase is a major parameter in motor function and serves as a powerful method to monitor and study kinesin activity under various experimental conditions. As part of its Cytoskeleton Motor Werks (CMW) line of research reagents, Cytoskeleton, Inc. has developed the Kinesin ELIPA™ (Enzyme Linked Inorganic Phosphate Assay) Biochem Kit. The assay is an adaptation of a method originally described by Webb for the measurement of glycerol kinase plus D-glyceraldehyde ATPase activity and for actin activated myosin ATPase (4). The assay is based upon an absorbance shift (330 - 360 nm) that occurs when 2-amino-6-mercapto-7-methylpurine ribonucleoside (MESG) is catalytically converted to 2-amino-6-mercapto-7-methyl purine in the presence of inorganic phosphate (Pi). The reaction is catalyzed by purine nucleoside phosphorylase (PNP). One molecule of inorganic phosphate will yield one molecule of 2-amino-6-mercapto-7-methyl purine in an essentially irreversible reaction (5). Thus, the absorbance at 360 nm is directly proportional to the amount of Pi generated in the kinesin ATPase reaction.

保存建议

厂家推荐常温运输，收到产品后建议保存于4°C。

其他

Cytoskeleton公司成立于1993年，专注于生物化学和细胞过程研究中的纯化蛋白和便捷试剂盒开发与生产。公司提供药物筛选、信号转导、蛋白质转录后修饰（PTM）、细胞骨架研究相关的系列试剂盒和产品，尤其以细胞骨架相关研究见长，既能满足于样品较少的科学研究，也可以用于小规模筛选研究和高通量大规模筛选研究。此外，公司还提供微管蛋白，肌动蛋白，小G蛋白，GAPs，GEFs等现有产品的药物筛选服务。