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网站主页 化工产品目录 生物化工 酶及辅酶类 糜蛋白酶 α-糜蛋白酶 来源于牛胰腺(TLCK处理)
  • aladdin 阿拉丁 C106197 α-糜蛋白酶 来源于猪胰脏 9004-07-3 800 usp u/mg

aladdin 阿拉丁 C106197 α-糜蛋白酶 来源于猪胰脏 9004-07-3 800 usp u/mg

α-Chymotrypsin from bovine pancreas(TLCK Treated)
9004-07-3
￥699.90 25mg 起订
￥1799.90 100mg 起订
上海 更新日期：2025-05-16

上海阿拉丁生化科技股份有限公司

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联系人：阿拉丁客服
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产品详情:

中文名称：
α-糜蛋白酶 来源于牛胰腺(TLCK处理)
英文名称：
α-Chymotrypsin from bovine pancreas(TLCK Treated)
CAS号：
9004-07-3
品牌：
阿拉丁
产地：
上海
保存条件：
2-8°C储存
纯度规格：
ActiBioPure™,EnzymoPure™,生物活性,天然,≥45 units/mg protein
产品类别：
小分子和化合物库 按“信号通路”分类
运输条件：
低温运输
产品规格：
100mg、25mg
货号：
C129079
是否进口：
否

基本描述

产品名称：α-糜蛋白酶 来源于牛胰腺(TLCK处理)

规格或纯度：ActiBioPure™,EnzymoPure™,生物活性,天然,≥45 units/mg protein

产品介绍：

Chymotrypsin preferentially catalyzes the hydrolysis of peptide bonds involving L-isomers of tyrosine, phenylalanine and tryptophan. It also readily acts upon amides and esters of susceptible amino acids. Chymotrypsin catalyzes the hydrolysis of bonds of leucyl, methionyl, asparaginyl and glutamyl residues.
Stability/Storage: The enzyme is stable for days in solution at pH 3.0 and for years as a dry powder at 2-8°C. Protect from moisture.
Characteristics of Chymotrypsin
Architecture: Beta Barrel
Topology: Trypsin-like Serine Protease
Molecular Weight：25.6 kDa
Optimal pH：7.8-8.0
Isoelectric point：
8.52 (Chymotrypsinogen, Theoretical)
8.33 (Chymotrypsin, Theoretical)
Extinction Coefficient：
51,840 cm-1 M-1 (Theoretical)
E1%,280 = 20.19 (Chymotrypsinogen, Theoretical)
E1%,280 = 20.57 (Chymotrypsin, Theoretical)
Active Site Residues: Histidine (H57), Aspartate (D102), Serine (S195)
Activators: Cetyltributylammonium bromide, Dodecyltrimethylammonium bromide, Hexadecyltrimethylammonium bromide, Tetrabutylammonium bromide
Inhibitors: Hydroxymethylpyrroles, Boronic acids, Courmarin derivatives, Peptidyl aldehydes, Peptides from natural sources, Peptides containing an unnatural amino acid
Chymotrypsin Assay
Method
The reaction velocity is determined according to Hummel (1959) by measuring an increase in absorbance at 256 nm resulting from the hydrolysis of benzoyl-L-tyrosine ethyl ester. One unit hydrolyzes one micromole of benzoyl-L-tyrosine ethyl ester (BTEE) per minute at pH 7.8 and 25°C under the specified conditions.
Reagents
0.08 M Tris⋅HCl buffer, pH 7.8 containing 0.1 M calcium chloride
0.00107 M Benzoyl-L-tyrosine ethyl ester (BTEE) in 50% w/w methanol (63 ml absolute methanol added to 50 ml reagent grade water)
0.001 N HCl
Enzyme
Dissolve enzyme at one mg/ml in 0.001 N HCl. Dilute in 0.001 N HCl to 10-30 μg/ml for assay.
Procedure
Adjust the spectrophotometer to 256 nm and 25°C.
Pipette into cuvettes as follows:
0.08 M Tris⋅HCl buffer, pH 7.8 with 0.1 M CaCl2 1.5 ml
0.00107 M BTEE 1.4 ml
Incubate in spectrophotometer at 25°C for 4-5 minutes to achieve temperature equilibrium and record blank rate, if any. Add 0.1 ml of appropriately diluted enzyme and record increase in absorbance at 256 nm for 4-5 minutes. Calculate ΔA256/min from the initial linear portion of the curve.
Calculation

where 964 is the extinction coefficient of BTEE at 256 nm.

Chymotrypsin preferentially catalyzes the hydrolysis of peptide bonds involving L-isomers of tyrosine, phenylalanine and tryptophan. It also readily acts upon amides and esters of susceptible amino acids. Chymotrypsin catalyzes the hydrolysis of bonds of leucyl, methionyl, asparaginyl and glutamyl residues.
Stability/Storage: The enzyme is stable for days in solution at pH 3.0 and for years as a dry powder at 2-8°C. Protect from moisture.
Characteristics of Chymotrypsin
Architecture: Beta Barrel
Topology: Trypsin-like Serine Protease
Molecular Weight：25.6 kDa
Optimal pH：7.8-8.0
Isoelectric point：
8.52 (Chymotrypsinogen, Theoretical)
8.33 (Chymotrypsin, Theoretical)
Extinction Coefficient：
51,840 cm-1 M-1 (Theoretical)
E1%,280 = 20.19 (Chymotrypsinogen, Theoretical)
E1%,280 = 20.57 (Chymotrypsin, Theoretical)
Active Site Residues: Histidine (H57), Aspartate (D102), Serine (S195)
Activators: Cetyltributylammonium bromide, Dodecyltrimethylammonium bromide, Hexadecyltrimethylammonium bromide, Tetrabutylammonium bromide
Inhibitors: Hydroxymethylpyrroles, Boronic acids, Courmarin derivatives, Peptidyl aldehydes, Peptides from natural sources, Peptides containing an unnatural amino acid
Chymotrypsin Assay
Method
The reaction velocity is determined according to Hummel (1959) by measuring an increase in absorbance at 256 nm resulting from the hydrolysis of benzoyl-L-tyrosine ethyl ester. One unit hydrolyzes one micromole of benzoyl-L-tyrosine ethyl ester (BTEE) per minute at pH 7.8 and 25°C under the specified conditions.
Reagents
0.08 M Tris⋅HCl buffer, pH 7.8 containing 0.1 M calcium chloride
0.00107 M Benzoyl-L-tyrosine ethyl ester (BTEE) in 50% w/w methanol (63 ml absolute methanol added to 50 ml reagent grade water)
0.001 N HCl
Enzyme
Dissolve enzyme at one mg/ml in 0.001 N HCl. Dilute in 0.001 N HCl to 10-30 μg/ml for assay.
Procedure
Adjust the spectrophotometer to 256 nm and 25°C.
Pipette into cuvettes as follows:
0.08 M Tris⋅HCl buffer, pH 7.8 with 0.1 M CaCl2 1.5 ml
0.00107 M BTEE 1.4 ml
Incubate in spectrophotometer at 25°C for 4-5 minutes to achieve temperature equilibrium and record blank rate, if any. Add 0.1 ml of appropriately diluted enzyme and record increase in absorbance at 256 nm for 4-5 minutes. Calculate ΔA256/min from the initial linear portion of the curve.
Calculation

where 964 is the extinction coefficient of BTEE at 256 nm.

生物活性：≥45 units/mg protein

Accession #：P00766,P00767

级别：生物活性, ActiBioPure™, 天然, EnzymoPure™


产品规格参数

浓度：≥45 units/mg protein

复溶：Reconstitute in 0.001 N HCl.

储存温度：2-8°C储存

运输条件：低温运输

CAS编号和信息：9004-07-3

酶学委员会编号：EC 3.4.21.1

单位定义：One Unit hydrolyzes one micromole of benzoyl-L-tyrosine ethyl ester per minute at 25°C, pH 7.8 in the presence of calcium. An activity of 45 units per mg using the above definition, is the equivalent of 10,000 optical density or 1330 N.F. units per mg usi


功能和特点

Accession #：P00766,P00767

生物活性：≥45 units/mg protein


查看阿拉丁官网此产品相关对应页面：https://www.aladdin-e.com/zh_cn/C129079.html


α-胰凝乳蛋白酶;N-Succinyl-Gly-Gly-Phe-p-nitroanilide, protease substrate ; Chymotrypsin Substrate I, Colorimetric ; N-(3-Carboxy-1-oxopropyl)glycylglycyl-N-(4-nitrophenyl)-L-phenylalaninamide

公司简介

上海阿拉丁生化科技股份有限公司是A股上市公司（（股票代码：688179），专注于科研试剂的研发、生产和销售，已陆续建立多个工厂和研发中心。作为领军企业，阿拉丁始终坚持质量第一，连续13年被评为“最受欢迎试剂品牌”。 阿拉丁目前常备库存试剂产品品种超过7万种，SKU总数超过46万，产品线涵盖了化学试剂、生化试剂、药靶配体、蛋白质和抗体等多个领域，是国内少数化学试剂到生物试剂全面发展的国产试剂品牌，产品同步发布在我们国内外电商平台。

成立日期 (18年)
注册资本 14130.676万人民币
员工人数 500人以上
年营业额 ￥ 1亿以上
经营模式 工厂,试剂
主营行业 生物化工,化学试剂

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