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网站主页 RECOMBINANT BAX ANTIBODY Recombinant Bax Antibody
  • aladdin 阿拉丁 Ab090652 Recombinant Bax Antibody Recombinant (RR647); Rabbit anti Human Bax Antibody; WB, IHC, Flow, IP; Unconjugated
  • aladdin 阿拉丁 Ab090652 Recombinant Bax Antibody Recombinant (RR647); Rabbit anti Human Bax Antibody; WB, IHC, Flow, IP; Unconjugated
  • aladdin 阿拉丁 Ab090652 Recombinant Bax Antibody Recombinant (RR647); Rabbit anti Human Bax Antibody; WB, IHC, Flow, IP; Unconjugated
  • aladdin 阿拉丁 Ab090652 Recombinant Bax Antibody Recombinant (RR647); Rabbit anti Human Bax Antibody; WB, IHC, Flow, IP; Unconjugated

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aladdin 阿拉丁 Ab090652 Recombinant Bax Antibody Recombinant (RR647); Rabbit anti Human Bax Antibody; WB, IHC, Flow, IP; Unconjugated

Recombinant Bax Antibody
1299.90 50μl 起订
2099.90 100μl 起订
上海 更新日期:2025-05-16

上海阿拉丁生化科技股份有限公司

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联系人:阿拉丁客服
电话:400-6206333拨打
手机:13167063860 拨打
邮箱:anhua.mao@aladdin-e.com

产品详情:

中文名称:
Recombinant Bax Antibody
英文名称:
Recombinant Bax Antibody
品牌:
阿拉丁
产地:
上海
保存条件:
-20°C储存,避免反复冻融
纯度规格:
ExactAb™, Validated, Recombinant, High performance, 0.25mg/mL
产品类别:
抗体 流式抗体
分子式:
C60H103N2O13S4
分子量:
1286.75
运输条件:
超低温运输
产品规格:
100μl、50μl
货号:
Ab090652
是否进口:

基本信息

产品名称:Recombinant Bax Antibody

规格或纯度:ExactAb™, Validated, Recombinant, High performance, 0.25mg/mL

应用:WB, IF/ICC, IP, Flow Cytometry, IHC

特异性:Bax

种属反应性:人(Human), 小鼠(Mouse), 大鼠(Rat)

免疫原:A synthetic peptide of human BAX (AA 1-100).

阳性对照:WB: A549, HT-29, HeLa, LNCaP, Hep G2 and HKE293 cell lysates. IHC: Human Ovarian cancer tissue. IF/ICC: HeLa cells. Flow: HeLa cells. IP: HT-29 whole cell lysate.

偶联:Unconjugated

备注:本产品即将售完下架,替代产品为 Ab090655


产品属性

克隆类型:重组抗体

克隆号:RR647

Format:Whole IgG

亚型:Rabbit IgG

SDS-PAGE:150 kDa

纯化方法:Protein A purified

物理外观:Liquid

储存缓冲液:59% 10mM PBS, 40% Glycerol, 0.05% BSA, 0.01% Sodium azide, pH 7.3.

防腐剂:0.01% Sodium azide

浓度:0.25mg/mL

储存温度:-20°C储存,避免反复冻融

运输条件:超低温运输

稳定性与储存:4°C 短期储存(1-2 周)。-20°C下长期保存(24个月)。收货后建议分装。避免冷冻/解冻循环。



功能和特点

宿主种属:兔(Rabbit)

种属反应性:人(Human), 小鼠(Mouse), 大鼠(Rat)

克隆号:RR647

偶联:Unconjugated

图片

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Western Blot: Recombinant Bax Antibody (Ab090652)

Recombinant Bax Antibody (Ab090652) - Western Blot
All lanes: Recombinant Bax Antibody (Ab090652) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/10000 dilution

Predicted band size: 21 kDa
Observed band size: 20 kDa
Exposure time: 6.2 s


>
IHC: Recombinant Bax Antibody (Ab090652)

Recombinant Bax Antibody (Ab090652) - IHC
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian cancer tissue labelling Bax with Recombinant Bax Antibody (Ab090652) at 1/1600. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9.0.

>
Flow Cytometry: Recombinant Bax Antibody (Ab090652)

Recombinant Bax Antibody (Ab090652) - Flow Cytometry
Overlay histogram showing HeLa cells stained with Recombinant Bax Antibody (Ab090652) (Red). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% TritonX-100 for 15 min. The cells were then incubated in the Recombinant Bax Antibody (Ab090652) (1/400 dilution) in 1x PBS/1% BSA for 30 min at room temperature. The secondary antibody was used a Goat Anti-Rabbit AF488 (IgG H+L) at 1/2000 dilution for 20 min at room temperature. Unlabeled sample (Black) was used as a control.

>
IP: Recombinant Bax Antibody (Ab090652)

Recombinant Bax Antibody (Ab090652) - IP
Bax was immunoprecipitated from 0.5 mg HT-29 whole cell lysate with Recombinant Bax Antibody (Ab090652) at 1/25 dilution.
2nd Ab: GAR HRP for IP 1/500

Lane 1: Recombinant Bax Antibody (Ab090652) IP in HT-29 whole cell lysate
Lane 2: PBS instead of Recombinant Bax Antibody (Ab090652) in HT-29 whole cell lysate
Lane 3: HT-29 whole cell lysate, 10 μg (input)

Exposure: 30 s.

>
IF/ICC: Recombinant Bax Antibody (Ab090652)

Recombinant Bax Antibody (Ab090652) - IF/ICC
IF analysis of Recombinant Bax Antibody (Ab090652) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 5% BSA for 1 hour at room temperature. Cells were stained with Recombinant Bax Antibody (Ab090652) at 1/1000 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (FITC) (Ab170148) at a dilution of 1/1000 for 1 hour at room temperature in the dark (green). Cells were counterstained with DAPI (blue).


查看阿拉丁官网此产品相关对应页面:https://www.aladdin-e.com/zh_cn/Ab090652.html


Bax抗体 ; anti- Bax抗体 ; Bcl2关联X蛋白抗体 ; 重组Bax抗体

公司简介

上海阿拉丁生化科技股份有限公司是A股上市公司((股票代码:688179),专注于科研试剂的研发、生产和销售,已陆续建立多个工厂和研发中心。作为领军企业,阿拉丁始终坚持质量第一,连续13年被评为“最受欢迎试剂品牌”。 阿拉丁目前常备库存试剂产品品种超过7万种,SKU总数超过46万,产品线涵盖了化学试剂、生化试剂、药靶配体、蛋白质和抗体等多个领域,是国内少数化学试剂到生物试剂全面发展的国产试剂品牌,产品同步发布在我们国内外电商平台。

成立日期 (18年)
注册资本 14130.676万人民币
员工人数 500人以上
年营业额 ¥ 1亿以上
经营模式 工厂,试剂
主营行业 生物化工,化学试剂

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