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网站主页 RECOMBINANT ERK1 ANTIBODY Recombinant ERK1 Antibody
  • aladdin 阿拉丁 Ab102223 Recombinant ERK1 Antibody Recombinant (RR671); Rabbit anti Human ERK1 Antibody; WB, IHC, Flow, IF/ICC, IP; Unconjugated
  • aladdin 阿拉丁 Ab102223 Recombinant ERK1 Antibody Recombinant (RR671); Rabbit anti Human ERK1 Antibody; WB, IHC, Flow, IF/ICC, IP; Unconjugated
  • aladdin 阿拉丁 Ab102223 Recombinant ERK1 Antibody Recombinant (RR671); Rabbit anti Human ERK1 Antibody; WB, IHC, Flow, IF/ICC, IP; Unconjugated
  • aladdin 阿拉丁 Ab102223 Recombinant ERK1 Antibody Recombinant (RR671); Rabbit anti Human ERK1 Antibody; WB, IHC, Flow, IF/ICC, IP; Unconjugated
  • aladdin 阿拉丁 Ab102223 Recombinant ERK1 Antibody Recombinant (RR671); Rabbit anti Human ERK1 Antibody; WB, IHC, Flow, IF/ICC, IP; Unconjugated

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aladdin 阿拉丁 Ab102223 Recombinant ERK1 Antibody Recombinant (RR671); Rabbit anti Human ERK1 Antibody; WB, IHC, Flow, IF/ICC, IP; Unconjugated

Recombinant ERK1 Antibody
￥499.90 10μl 起订
￥1299.90 50μl 起订
￥2099.90 100μl 起订
上海 更新日期：2025-05-16

上海阿拉丁生化科技股份有限公司

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联系人：阿拉丁客服
电话：400-6206333拨打
手机：13167063860 拨打
邮箱：anhua.mao@aladdin-e.com

产品详情:

中文名称：
Recombinant ERK1 Antibody
英文名称：
Recombinant ERK1 Antibody
品牌：
阿拉丁
产地：
上海
保存条件：
-20°C储存,避免反复冻融
纯度规格：
ExactAb™, Validated, Recombinant, High performance, 2mg/mL
产品类别：
抗体 流式抗体
分子式：
C60H103N2O13S4
分子量：
1286.75
运输条件：
超低温运输
用途：
WB,Flow,IF,ICC,IHC,IP
产品规格：
100μl、50μl、10μl
货号：
Ab102223
是否进口：
否

基本信息

产品名称：Recombinant ERK1 Antibody

规格或纯度：ExactAb™, Validated, Recombinant, High performance, 2mg/mL

应用：WB, IF/ICC, IP, Flow Cytometry, IHC

特异性：MAPK3

应用：WB,Flow,IF,ICC,IHC,IP

种属反应性：人(Human), 小鼠(Mouse), 大鼠(Rat)

交叉反应：Cross-reactivity with other species has not been measured.

免疫原：A synthetic peptide corresponding to the N-term of ERK1 was used as an immunogen.

阳性对照：WB: HeLa, A431, NIH/3T3, Jurkat and K562 cell lysates. IHC: Cervix cancer tissue. Flow: HeLa cells. ICC/IF: HeLa cells. IP: A375 whole cell lysate.

偶联：Unconjugated


产品属性

抗体类型：Primary antibody

克隆类型：重组抗体

克隆号：RR671

Format：Whole IgG

亚型：Rabbit IgG

SDS-PAGE：150 kDa

纯化方法：Protein A purified

来源：CHO supernatant

物理外观：Liquid

储存缓冲液：1*PBS 59%, Sodium azide 0.01%, Glycerol 40%, BSA 0.05%, pH7.3.

防腐剂：0.01% Sodium azide

浓度：2mg/mL

储存温度：-20°C储存,避免反复冻融

运输条件：超低温运输

稳定性与储存：在 -20°C 下可保存 2 年。收货后建议分装。避免冷冻/解冻循环。



功能和特点

宿主种属：兔(Rabbit)

种属反应性：人(Human), 小鼠(Mouse), 大鼠(Rat)

克隆号：RR671

偶联：Unconjugated

图片

>
Western Blot: Recombinant ERK1 Antibody (Ab102223)

Recombinant ERK1 Antibody (Ab102223) - Western Blot
All lanes: Recombinant ERK1 Antibody (Ab102223) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/30000 dilution

Predicted band size: 43 kDa
Observed band size: 40 kDa
Exposure time: 27.0 s

>
IF/ICC: Recombinant ERK1 Antibody (Ab102223)

Recombinant ERK1 Antibody (Ab102223) - IF/ICC
Recombinant ERK1 Antibody (Ab102223) staining ERK1 in HeLa cells by IF/ICC (immunofluorescence/immunocytochemistry). Cells were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100 and blocked with 10% goat serum for half an hour at room temperature. Samples were incubated with Recombinant ERK1 Antibody (Ab102223) (1/50) at 4°C. An AF488-conjugatedGoat Anti-Rabbit IgG polyclonal was used as the secondary antibody (1/500). DAPI (blue) was used as the nuclear counter stain.

Control: PBS and secondary antibody, An AF488-conjugatedGoat Anti-Rabbit IgG (1/500).

>
Flow Cytometry: Recombinant ERK1 Antibody (Ab102223)

Recombinant ERK1 Antibody (Ab102223) - Flow Cytometry
Overlay histogram showing HeLa cells stained with Recombinant ERK1 Antibody (Ab102223) (Red). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% TritonX-100 for 15 min. The cells were then incubated in the Recombinant ERK1 Antibody (Ab102223) (1/1000 dilution) in 1x PBS/1% BSA for 30 min at room temperature. The secondary antibody was used a Goat Anti-Rabbit AF488 (IgG H+L) at 1/2000 dilution for 20 min at room temperature. Unlabeled sample (Black) was used as a control.

>
IP: Recombinant ERK1 Antibody (Ab102223)

Recombinant ERK1 Antibody (Ab102223) - IP
ERK1 was immunoprecipitated from 0.4 mg of A375 whole cell lysate with Recombinant ERK1 Antibody (Ab102223) at 1/50 dilution
2nd Ab: GAR HRP for IP 1/500

Lane 1: Recombinant ERK1 Antibody (Ab102223) IP in A375 whole cell lysate
Lane 2: PBS instead of Recombinant ERK1 Antibody (Ab102223) in A375 whole cell lysate
Lane 3: A375 whole cell lysate, 10 μg (input)

Exposure: 120.0 s.

>
IHC: Recombinant ERK1 Antibody (Ab102223)

Recombinant ERK1 Antibody (Ab102223) - IHC
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded
sections) analysis Cervix cancer tissue labelling ERK1 with Recombinant ERK1 Antibody (Ab102223) at 1:200. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9.0.


查看阿拉丁官网此产品相关对应页面：https://www.aladdin-e.com/zh_cn/Ab102223.html


ERK1抗体 ; ERK1兔抗体 ; ERK1单克隆抗体 ; 重组ERK1抗体

公司简介

上海阿拉丁生化科技股份有限公司是A股上市公司（（股票代码：688179），专注于科研试剂的研发、生产和销售，已陆续建立多个工厂和研发中心。作为领军企业，阿拉丁始终坚持质量第一，连续13年被评为“最受欢迎试剂品牌”。 阿拉丁目前常备库存试剂产品品种超过7万种，SKU总数超过46万，产品线涵盖了化学试剂、生化试剂、药靶配体、蛋白质和抗体等多个领域，是国内少数化学试剂到生物试剂全面发展的国产试剂品牌，产品同步发布在我们国内外电商平台。

成立日期 (18年)
注册资本 14130.676万人民币
员工人数 500人以上
年营业额 ￥ 1亿以上
经营模式 工厂,试剂
主营行业 生物化工,化学试剂

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