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网站主页 RECOMBINANT GAPDH ANTIBODY Recombinant GAPDH Antibody
  • aladdin 阿拉丁 Ab104788 Recombinant GAPDH Antibody Recombinant (RR688); Rabbit anti Human GAPDH Antibody; WB, IHC, Flow, IF, ICC, IP; Unconjugated
  • aladdin 阿拉丁 Ab104788 Recombinant GAPDH Antibody Recombinant (RR688); Rabbit anti Human GAPDH Antibody; WB, IHC, Flow, IF, ICC, IP; Unconjugated
  • aladdin 阿拉丁 Ab104788 Recombinant GAPDH Antibody Recombinant (RR688); Rabbit anti Human GAPDH Antibody; WB, IHC, Flow, IF, ICC, IP; Unconjugated
  • aladdin 阿拉丁 Ab104788 Recombinant GAPDH Antibody Recombinant (RR688); Rabbit anti Human GAPDH Antibody; WB, IHC, Flow, IF, ICC, IP; Unconjugated
  • aladdin 阿拉丁 Ab104788 Recombinant GAPDH Antibody Recombinant (RR688); Rabbit anti Human GAPDH Antibody; WB, IHC, Flow, IF, ICC, IP; Unconjugated

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aladdin 阿拉丁 Ab104788 Recombinant GAPDH Antibody Recombinant (RR688); Rabbit anti Human GAPDH Antibody; WB, IHC, Flow, IF, ICC, IP; Unconjugated

Recombinant GAPDH Antibody
￥199.90 10μl 起订
￥599.90 50μl 起订
￥929.90 100μl 起订
上海 更新日期：2025-05-16

上海阿拉丁生化科技股份有限公司

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联系人：阿拉丁客服
电话：400-6206333拨打
手机：13167063860 拨打
邮箱：anhua.mao@aladdin-e.com

产品详情:

中文名称：
Recombinant GAPDH Antibody
英文名称：
Recombinant GAPDH Antibody
品牌：
阿拉丁
产地：
上海
保存条件：
-20°C储存,避免反复冻融
纯度规格：
ExactAb™, Validated, Recombinant, High performance, 0.5 mg/mL
产品类别：
抗体 流式抗体
分子式：
C60H103N2O13S4
分子量：
1286.75
运输条件：
超低温运输
产品规格：
100μl、50μl、10μl
货号：
Ab104788
是否进口：
否

基本信息

产品名称：Recombinant GAPDH Antibody

规格或纯度：ExactAb™, Validated, Recombinant, High performance, 0.5 mg/mL

应用：WB, IF/ICC, IP, Flow Cytometry, IHC

特异性：GAPDH

种属反应性：人(Human), 小鼠(Mouse), 大鼠(Rat), 非洲绿猴(African green monkey), 鸡(Chicken), 牛(Cow), 猪(Pig), 斑马鱼(Zebrafish), 绿猴(Green Monkey), 非洲爪蟾(Xenopus africanus)

交叉反应：Cross-reactivity with other species has not been measured.

免疫原：A synthetic peptide corresponding to residues of human GAPDH was used as an immunogen (AA 200-300).

阳性对照：WB: A431, MCF-7, A549, HEK293, HeLa, Raji, Jurkat, HUVEC, NIH/3T3 and C6 cell lysates. IHC: Prostate cancer tissue. Flow: HeLa cells. IF/ICC: HeLa cells. IP: HeLa whole cell lysate.

偶联：Unconjugated


产品属性

克隆类型：重组抗体

克隆号：RR688

Format：Whole IgG

亚型：Rabbit IgG

SDS-PAGE：150 kDa

纯化方法：Protein A purified

物理外观：Liquid

储存缓冲液：59% 10mM PBS, 40% Glycerol, 0.05% BSA, 0.01% Sodium azide, pH 7.3

防腐剂：0.01% Sodium azide

浓度：0.5 mg/mL

储存温度：-20°C储存,避免反复冻融

运输条件：超低温运输

稳定性与储存：4°C 短期储存（1-2 周）。-20°C下长期保存（24个月）。收货后建议分装。避免冷冻/解冻循环。



功能和特点

宿主种属：兔(Rabbit)

种属反应性：人(Human), 小鼠(Mouse), 大鼠(Rat), 非洲绿猴(African green monkey), 鸡(Chicken), 牛(Cow), 猪(Pig), 斑马鱼(Zebrafish), 绿猴(Green Monkey), 非洲爪蟾(Xenopus africanus)

克隆号：RR688

偶联：Unconjugated

图片

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Western Blot: Recombinant GAPDH Antibody (Ab104788)

Recombinant GAPDH Antibody (Ab104788) - Western Blot
All lanes: Recombinant GAPDH Antibody (Ab104788) at 1/5000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/30000 dilution

Predicted band size: 36 kDa
Observed band size: 35 kDa
Exposure time: 1.0 s

>
IF: Recombinant GAPDH Antibody (Ab104788)

Recombinant GAPDH Antibody (Ab104788) - IF
IF analysis of GAPDH (green) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained Recombinant GAPDH Antibody (Ab104788) at 1/100 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (AF488) at a dilution of 1/1000 for 1 hour at room temperature in the dark (green). Cells were counterstained with DAPI (blue). Images were taken on the confocal laser scanning microscope.

>
IHC: Recombinant GAPDH Antibody (Ab104788)

Recombinant GAPDH Antibody (Ab104788) - IHC
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of prostate cancer tissue labelling GAPDH with Recombinant GAPDH Antibody (Ab104788) at 1/1600. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9.0.

>
Flow Cytometry: Recombinant GAPDH Antibody (Ab104788)

Recombinant GAPDH Antibody (Ab104788) - Flow Cytometry
Overlay histogram showing HeLa cells stained with Recombinant GAPDH Antibody (Ab104788) (Red). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% TritonX-100 for 15 min. The cells were then incubated in Recombinant GAPDH Antibody (Ab104788) (1/1000 dilution) in 1x PBS/1% BSA for 30 min at room temperature. The secondary antibody was used a Goat Anti-Rabbit AF488 (IgG H+L) at 1/2000 dilution for 20 min at room temperature. Unlabelled sample (Black) was used as a control.

>
IP: Recombinant GAPDH Antibody (Ab104788)

Recombinant GAPDH Antibody (Ab104788) - IP
GAPDH was immunoprecipitated from 0.1 mg of HeLa whole cell lysate with Recombinant GAPDH Antibody (Ab104788) at 1/50 dilution.
2nd Ab: GAR HRP for IP 1/500

Lane 1: Recombinant GAPDH Antibody (Ab104788) IP in HeLa whole cell lysate
Lane 2: PBS instead of Recombinant GAPDH Antibody (Ab104788) in HeLa whole cell lysate
Lane 3: HeLa whole cell lysate, 2 μg (input).


查看阿拉丁官网此产品相关对应页面：https://www.aladdin-e.com/zh_cn/Ab104788.html


3-磷酸甘油醛脱氢酶(内参)抗体 ; 3-磷酸甘油醛脱氢酶抗体 ; 重组GAPDH抗体;38 kDa BFA-dependent ADP-ribosylation substrate antibody

公司简介

上海阿拉丁生化科技股份有限公司是A股上市公司（（股票代码：688179），专注于科研试剂的研发、生产和销售，已陆续建立多个工厂和研发中心。作为领军企业，阿拉丁始终坚持质量第一，连续13年被评为“最受欢迎试剂品牌”。 阿拉丁目前常备库存试剂产品品种超过7万种，SKU总数超过46万，产品线涵盖了化学试剂、生化试剂、药靶配体、蛋白质和抗体等多个领域，是国内少数化学试剂到生物试剂全面发展的国产试剂品牌，产品同步发布在我们国内外电商平台。

成立日期 (18年)
注册资本 14130.676万人民币
员工人数 500人以上
年营业额 ￥ 1亿以上
经营模式 工厂,试剂
主营行业 生物化工,化学试剂

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