beta Actin Mouse mAb (Biotin) (Ab175850) - IF
IF analysis of beta Actin (red) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with beta Actin Mouse mAb (Biotin) (Ab175850) at 1/2000 dilution in blocking buffer overnight at 4°C. Then incubated with Recombinant Streptavidin Protein (Cy5) (rp169047) at a dilution of 1/1000 for 1 hour at room temperature in the dark (red). Cells were counterstained with DAPI (blue). Images were taken on the confocal laser scanning microscope.

beta Actin Mouse mAb (Biotin) (Ab175850) - Western Blot
All lanes: beta Actin Mouse mAb (Biotin) (Ab175850) at 1/5000 dilution
Samples: Lysates at 20 µg per lane
SA-HRP: 1:20000

Predicted band size: 42.0 kDa
Observed band size: 42.0 kDa
Exposure time: 12.1s

beta Actin Mouse mAb (Biotin) (Ab175850) - Flow Cytometry
Flow cytometry analysis of Beta Actin in Hela cells (red) compared to an isotype control (blue). The cells were fixed and permeabilized with 100% methanol for 5 minutes. Cells were stained with beta Actin Mouse mAb (Biotin) (Ab175850) at 1/100 dilution for 1 hour at 4°C. Then incubated with Recombinant Streptavidin Protein (Cy5) (rp169047) at a dilution of 1/2000 for 30 minutes at 4°C in the dark.