小鼠LRRC32&TGF-beta 1蛋白-ACROBiosystems百普赛斯

Mouse LRRC32&TGF-beta 1 Heterotrimer protein, His Tag&Tag Free (MALS verified) 

分子别名（Synonym）

LRRC32 & TGF-beta 1,LRRC32&TGFB1

表达区间及表达系统（Source）

Mouse LRRC32&TGFB1 Heterotrimer protein, His Tag&Tag Free (GA1-M52W2) is expressed from human 293 cells (HEK293). It contains AA Ile 18 - Asn 628 (LRRC32) & Leu 30 - Ser 390 (TGF-beta 1) (Accession # G3XA59-1 (LRRC32) & P04202-1 (TGF-beta 1)).

Predicted N-terminus: Leu 30

蛋白结构（Molecular Characterization）

Mouse LRRC32&TGFB1 Heterotrimer Protein, His Tag&Tag Free (the molar ratio of LRRC32 & TGF-beta 1 equals 1:2) is produced by co-expression of LRRC32 and TGF-beta 1, which has a calculated MW of 68.7 kDa (LRRC32), 28.5 (LAP) and 12.8 kDa (mature TGF-beta 1) respectively. LRRC32 is fused with a polyhistidine tag at the C-terminus and TGF-beta 1 contains no tag. The reducing (R) Heterotrimer protein migrates as 80 kDa (LRRC32), 40 kDa (LAP) and 14 kDa (mature TGF-beta 1) due to glycosylation respectively.

内毒素（Endotoxin）

Less than 1.0 EU per μg by the LAL method.

纯度（Purity）

>95% as determined by SDS-PAGE.

>90% as determined by SEC-MALS.

制剂（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

背景（Background）

GARP（LRRC32）是一种跨膜蛋白，结合潜在的TGF-β1并将其固定在Treg表面。并已被证明可以促进转化生长因子β（TGF-β）的活化和分泌。GARP在表面活化的Tregs上高度表达，并增强Tregs的抑制功能。此外，GARP可以与潜在的转化生长因子β（TGF-β）结合，从而促进TGF-β的分泌和激活。TGF-β对Tregs的稳态和功能起着关键作用。值得注意的是，还观察到表达GARP/潜在TGF-β1复合物的成纤维细胞和内皮细胞系不会激活TGF-β1。然而，不能排除需要特定的刺激来触发这些细胞类型表面复合物的TGF-β1激活。