CDH16抗体

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     Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);    

  
  

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     Western blot analysis using CDH16 mouse mAb against A431 (1), Jurkat (2) cell lysate.    

  
  

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     Immunofluorescence analysis of A431 cells using CDH16 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)    

  
  

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     Flow cytometric analysis of A431 cells using CDH16 mouse mAb (green) and negative control (red).    

  
  

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     Immunohistochemical analysis of paraffin-embedded human kidney tissues using CDH16 mouse mAb with DAB staining.    

  
  

The CDH16 antibody targets cadherin-16 (CDH16), a calcium-dependent cell adhesion protein belonging to the cadherin superfamily. CDH16. also known as kidney-specific cadherin (KSP-cadherin) or human hepatocarcinoma-intestine-pancreas/pancreatitis-associated protein (HIP/PAP), is primarily expressed in the kidney, specifically in proximal tubule epithelial cells, and in the thyroid gland. It plays a critical role in maintaining tissue architecture by mediating cell-cell adhesion and polarity. CDH16's restricted expression makes it a valuable biomarker for studying kidney and thyroid development, function, and associated pathologies.

In research, CDH16 antibodies are widely used to identify and characterize renal proximal tubular cells in immunohistochemistry, flow cytometry, and Western blotting. They also aid in distinguishing kidney-derived tumors (e.g., renal cell carcinomas) from other malignancies due to CDH16's tissue specificity. Additionally, CDH16 has been implicated in developmental studies, as its expression correlates with nephron formation and renal epithelial differentiation. Dysregulation of CDH16 is associated with kidney diseases and thyroid disorders, though its exact mechanistic roles remain under investigation.

Structurally, CDH16 contains characteristic cadherin repeats and a conserved cytoplasmic domain that interacts with catenins, linking it to the cytoskeleton. The antibody’s utility in both basic and clinical research underscores its importance in understanding cellular adhesion mechanisms and tissue-specific pathologies.