RPS6KA2抗体

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     Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);    

  
  

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     Western blot analysis using RPS6KA2 mouse mAb against Hela (1), A431 (2), HEK293 (3), Jurkat (4), HepG2 (5), MCF-7 (6), NIH/3T3 (7) cell lysate.    

  
  

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     Immunofluorescence analysis of A431 cells using RPS6KA2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)    

  
  

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     Flow cytometric analysis of Hela cells using RPS6KA2 mouse mAb (green) and negative control (red).    

  
  

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     Immunohistochemical analysis of paraffin-embedded human cervical cancer tissues using RPS6KA2 mouse mAb with DAB staining.    

  
  

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     Immunohistochemical analysis of paraffin-embedded human lung cancer tissues using RPS6KA2 mouse mAb with DAB staining.    

  
  

The RPS6KA2 antibody targets the ribosomal protein S6 kinase A2 (RPS6KA2), also known as mitogen- and stress-activated protein kinase 1 (MSK1) or MAPKAP-K1. This serine/threonine kinase is activated by the MAPK signaling pathways, including ERK and p38. in response to growth factors, stress, or cytokines. RPS6KA2 phosphorylates substrates like CREB and NF-κB, regulating gene transcription linked to cell proliferation, survival, differentiation, and inflammatory responses. Structurally, it contains two kinase domains and a C-terminal regulatory region, enabling interactions with signaling complexes.

RPS6KA2 is implicated in neurological development and disorders, with studies associating its dysfunction with autism spectrum disorders, schizophrenia, and intellectual disability. It also plays roles in cancer, where its dual pro- or anti-tumor effects depend on cellular context. The RPS6KA2 antibody is widely used in research to detect protein expression, localization, and activation in tissues or cultured cells via techniques like Western blot, immunohistochemistry, and immunofluorescence. Its application aids in elucidating RPS6KA2's involvement in disease mechanisms and potential therapeutic targeting. This antibody serves as a critical tool for exploring kinase-driven signaling networks and their pathological implications.