ULK2抗体

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     Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)    

  
  

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     Western blot analysis using ULK2 mouse mAb against NIH/3T3 (1), HepG2 (2), SK-Hep-1 (3), SK-OV-3 (4), C6 (5), PC-12 (6), and MCF-7 (7) cell lysate.    

  
  

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     Flow cytometric analysis of Hela cells using ULK2 mouse mAb (green) and negative control (red).    

  
  

The ULK2 antibody is a research tool designed to detect Unc-51-like kinase 2 (ULK2), a serine/threonine kinase critical in autophagy regulation. ULK2. homologous to ULK1. forms complexes with autophagy-related proteins (ATG13. FIP200. ATG101) to initiate autophagosome formation. It integrates stress signals via upstream regulators like mTORC1 and AMPK, which phosphorylate ULK2 to modulate its activity. While ULK1 is dominant in autophagy, ULK2 exhibits overlapping functions and tissue-specific roles, particularly in contexts where ULK1 is absent or dysregulated.

ULK2 antibodies are widely used in techniques like Western blotting, immunofluorescence, and immunohistochemistry to assess protein expression, localization, and post-translational modifications (e.g., phosphorylation at Ser757 or Ser758). These antibodies often target specific epitopes, such as the N-terminal kinase domain or C-terminal region. Validation includes knockout/knockdown controls to confirm specificity.

Research applications span cancer, neurodegeneration, and metabolic disorders, as ULK2 dysregulation links to autophagy-related pathologies. Commercial ULK2 antibodies vary in host species (rabbit, mouse), clonality (monoclonal/polyclonal), and reactivity (human, mouse, rat). Researchers must select antibodies based on experimental needs, emphasizing validation data to ensure reliability. Understanding ULK2's role enhances insights into autophagy mechanisms and therapeutic targeting in disease.