TFDP1抗体

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     Gel: 8%SDS-PAGE, Lysate: 40 μg, Lane: NIH/3T3 cells, Primary antibody: P01299(TFDP1 Antibody) at dilution 1/650, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 3 minutes    

  
  

The TFDP1 (Transcription Factor Dp-1) antibody is a tool used to detect and study the DP1 protein, a key regulatory component in cell cycle progression and gene expression. TFDP1 belongs to the DP family of transcription factors and forms heterodimers with E2F proteins (E2F1-E2F6), enabling their DNA-binding activity. These complexes regulate the transcription of genes involved in cell cycle transitions, DNA replication, and apoptosis. Dysregulation of TFDP1-E2F interactions is linked to cancer, as aberrant cell proliferation and survival are hallmarks of tumorigenesis.

Researchers use TFDP1 antibodies in techniques like Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF) to assess protein expression levels, localization, and interactions in cellular and tissue samples. Studies have shown that TFDP1 overexpression or altered activity is associated with various cancers, including hepatocellular carcinoma, breast cancer, and lung cancer, making it a potential biomarker or therapeutic target.

Commercially available TFDP1 antibodies are typically raised in rabbits or mice, targeting specific epitopes within the protein. Validation includes testing in knockout cell lines or tissues to confirm specificity. The antibody’s utility extends to both basic research—elucidating E2F/DP-mediated transcriptional mechanisms—and clinical investigations exploring its role in disease progression and treatment response.