TP53抗体—艾普蒂

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     Gel: 8%SDS-PAGE, Lysate: 40 μg, Lane 1-4: HT29, HepG2, Hela, 231 cell lysates, Primary antibody: P06997(TP53 Antibody) at dilution 1/400, Secondary antibody: Goat anti rabbit IgG at 1/5000 dilution, Exposure time: 1 minute    

  
  

The TP53 antibody is a crucial tool in cancer research and diagnostics, targeting the p53 protein encoded by the TP53 gene. This gene, located on chromosome 17p13.1. functions as a tumor suppressor by regulating cell cycle arrest, DNA repair, apoptosis, and genomic stability. Mutations in TP53 are among the most common genetic alterations in human cancers, leading to dysfunctional p53 protein accumulation or loss of function, which promotes tumor progression.

TP53 antibodies are widely used in immunohistochemistry (IHC), Western blotting, and flow cytometry to detect p53 expression levels, localization, and mutation status. Wild-type p53 is typically short-lived and difficult to detect, whereas mutant p53 often accumulates in tumor cells due to altered degradation. Thus, strong nuclear staining in IHC may indicate TP53 mutations, though confirmatory genetic testing is required. These antibodies also aid in studying p53's role in cellular stress responses, therapeutic resistance, and interactions with other oncogenic pathways.

Clinically, TP53 antibody-based assays help stratify cancer subtypes, assess prognosis, and guide treatment strategies. However, interpretation requires caution due to variability in mutation types and antibody specificity. Ongoing research focuses on improving antibody sensitivity and developing therapies targeting mutant p53. Overall, TP53 antibodies remain indispensable for understanding cancer biology and advancing precision medicine.